Accuri C6 Flow Cytometer System
Originally published online as doi:10.1189/jlb.1207831 on March 27, 2008

Published online before print March 27, 2008
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(Journal of Leukocyte Biology. 2008;83:1459-1466.)
© 2008 by Society for Leukocyte Biology

Novel insights into the inhibitory effects of Galectin-1 on neutrophil recruitment under flow

Dianne Cooper1, Lucy V. Norling and Mauro Perretti

William Harvey Research Institute, Barts and The London, London, United Kingdom

1Correspondence: William Harvey Research Institute, Barts and The London, School of Medicine and Dentistry, Charterhouse Square, London EC1M 6BQ, UK. E-mail: d.cooper{at}qmul.ac.uk

Galectin-1 (Gal-1) is a β-galactoside-binding protein endowed with anti-inflammatory properties. The purpose of this study was to investigate the effects of endogenous and exogenous Gal-1 on neutrophil recruitment onto TNF-treated endothelium. The effect of human recombinant (hr)Gal-1 on markers of neutrophil activation (CD11b expression, P-selectin glycoprotein ligand 1, and L-selectin shedding) was also assessed. Gal-1 inhibited the platelet-activating factor-induced increase in CD11b expression in a concentration-dependent manner, as assessed by flow cytometry. To determine the effects of Gal-1 on neutrophil recruitment, an in vitro flow chamber was used: Preincubation of neutrophils with hrGal-1 significantly decreased the extent of capture, rolling, and adhesion on activated endothelial monolayers. This inhibition was shared with the endogenous protein, as knockdown of endothelial Gal-1 using small interfering RNA resulted in a significant increase in the number of cells captured and rolling. To verify the effects of Gal-1 in an in vivo system, intravital microscopy of Gal-1 null mice and their wild-type counterparts was performed. Leukocyte adhesion and emigration were increased significantly in the cremasteric circulation of Gal-1 null mice inflamed with IL-1β. These findings indicate that Gal-1 functions to limit neutrophil recruitment onto a TNF-treated endothelium, a property that may underline its inhibitory effects in acute inflammation.

Key Words: endothelial cells • inflammation • adhesion molecules




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L. V Norling, M. Perretti, and D. Cooper
Endogenous galectins and the control of the host inflammatory response
J. Endocrinol., May 1, 2009; 201(2): 169 - 184.
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