Journal of Leukocyte Biology eBioscience full spectrum cell analysis
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Originally published online as doi:10.1189/jlb.1107755 on February 12, 2008

Published online before print February 12, 2008
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(Journal of Leukocyte Biology. 2008;83:1145-1154.)
© 2008 by Society for Leukocyte Biology

Identification of CD4int progenitors in mouse fetal spleen, a source of resident lymphoid cells

Guillaume E. Desanti, Ana Cumano and Rachel Golub1

Unité du Développement des Lymphocytes, INSERM U668, Institut Pasteur, Paris Cedex, France

1Correspondence: Unité du Développement des Lymphocytes, INSERM U668, Institut Pasteur, 25, rue du Dr. Roux, 75724 Paris Cedex 15, France. E-mail: rgolub{at}pasteur.fr

Hematopoiesis occurs in different tissues during adult and fetal life. Splenic hematopoiesis arises in the fetal period until the first weeks of life. We have analyzed the hematopoietic progenitor content of the fetal spleen (FS) at the embryonic days 14.5–15.5. We first demonstrate that the hematopoietic content of the FS differs largely from its fetal liver (FL) counterpart. The difference mainly concerns the distribution of the different pool of progenitors, as most of the splenic progenitors are comprised in the lineage–Sca1–cKitlo contrary to the FL. We have divided the fetal hematopoietic pool into smaller fractions to enable characterization of the earliest lymphoid progenitors. Among the lymphoid progenitors that already represent a rare population, we were able to separate a population, respectively, enriched in B or T/NK progenitors. Lineage restriction of the different developmental intermediates was tested by clonal assays. We propose a model for fetal splenic hematopoietic progenitors and their distribution.

Key Words: hematopoiesis • cell differentiation • fetal development.






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