science pharmaceutical expo biotech jobs
Originally published online as doi:10.1189/jlb.1207810 on February 25, 2008

Published online before print February 25, 2008
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jlb.1207810v1
83/5/1100    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Franzoso, S.
Right arrow Articles by Papini, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Franzoso, S.
Right arrow Articles by Papini, E.
(Journal of Leukocyte Biology. 2008;83:1100-1110.)
© 2008 by Society for Leukocyte Biology

Human monocytes/macrophages are a target of Neisseria meningitidis Adhesin A (NadA)

Susanna Franzoso*, Cristina Mazzon*, Maryta Sztukowska{dagger}, Paola Cecchini*, Tihana Kasic{ddagger}, Barbara Capecchi§, Regina Tavano* and Emanuele Papini*,1

* Centro Ricerche Biotecnologie Innovative and
{ddagger} Istituto Veneto Medicina Molecolare, Dipartimento di Scienze Biomediche Sperimentali, Università di Padova, Padova, Italy;
{dagger} Rzeszów University of Technology, Faculty of Chemistry, Department of Biochemistry and Biotechnology, Rzeszów, Poland; and
§ Research Centre, Novartis Vaccines and Diagnostics S.r.l., Siena, Italy

1Correspondence: C.R.I.B.I.-University of Padova, Via G. Colombo 3, 35121 Padova, Italy. E-mail: emanuele.papini{at}unipd.it

ABSTRACT

Specific surface proteins of Neisseria meningitidis have been proposed to stimulate leukocytes during tissue invasion and septic shock. In this study, we demonstrate that the adhesin N. meningitidis Adhesin A (NadA) involved in the colonization of the respiratory epithelium by hypervirulent N. meningitidis B strains also binds to and activates human monocytes/macrophages. Expression of NadA on the surface on Escherichia coli does not increase bacterial-monocyte association, but a NadA-positive strain induced a significantly higher amount of TNF-{alpha} and IL-8 compared with the parental NadA-negative strain, suggesting that NadA has an intrinsic stimulatory action on these cells. Consistently, highly pure, soluble NadA{Delta}351–405, a proposed component of an antimeningococcal vaccine, efficiently stimulates monocytes/macrophages to secrete a selected pattern of cytokines and chemotactic factors characterized by high levels of IL-8, IL-6, MCP-1, and MIP-1{alpha} and low levels of the main vasoactive mediators TNF-{alpha} and IL-1. NadA{Delta}351–405 also inhibited monocyte apoptosis and determined its differentiation into a macrophage-like phenotype.

Key Words: adhesion molecule • bacterial infection • inflammation • cytokines • chemokines




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
R. Tavano, S. Franzoso, P. Cecchini, E. Cartocci, F. Oriente, B. Arico, and E. Papini
The membrane expression of Neisseria meningitidis adhesin A (NadA) increases the proimmune effects of MenB OMVs on human macrophages, compared with NadA- OMVs, without further stimulating their proinflammatory activity on circulating monocytes
J. Leukoc. Biol., July 1, 2009; 86(1): 143 - 153.
[Abstract] [Full Text] [PDF]