Journal of Leukocyte Biology Myeloid cells, immune suppression, tumor immunology
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Originally published online as doi:10.1189/jlb.0707492 on October 24, 2007 Originally published online as doi:10.1189/jlb.0707492 on October 15, 2007

Published online before print October 15, 2007
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(Journal of Leukocyte Biology. 2008;83:181-189.)
© 2008 by Society for Leukocyte Biology

Neutrophil granulocytes uniquely express, among human blood cells, high levels of Methionine-sulfoxide-reductase enzymes

Cesare Achilli, Annarita Ciana, Antonio Rossi, Cesare Balduini and Giampaolo Minetti1

University of Pavia, Department of Biochemistry, Pavia, Italy

1 Correspondence: University of Pavia, Department of Biochemistry, via Bassi 21, 27100 Pavia, Italy. E-mail: minetti{at}unipv.it

L-Methionine (Met), in its free form or when inserted in proteins, is sensitive to oxidation of its thioether group by reactive oxygen species from exogenous or endogenous sources. Two stable diastereomers of Met sulfoxide [Met-(O)] may be formed [Met-S-(O) and Met-R-(O)], but these can be reduced by two classes of Methionine-sulfoxide-reductase (Msr) enzymes: MsrA, which reduces the S, and MsrB, which reduces the R sulfoxide. In this study, we have examined the levels of expression of Msr in human blood cells by enzymatic activity assay, Western blotting, and RT-PCR of purified populations of polymorphonuclear neutrophils and eosinophils, mononuclear cells, platelets, and erythrocytes. Our data indicate that of the blood cells analyzed, neutrophils expressed the highest activity, which was mainly of MsrB type. During degranulation of activated neutrophils, Msr activity was not released but remained confined within the cell, indicating a non-granular localization. Immunoprecipitation and RT-PCR studies indicated the almost complete lack of mitochondrial forms of Msrs in granulocytes. It is thus likely that Msrs are important as antioxidant/repair systems for neutrophils, cells with enormous capacity for the generation of reactive oxidants and hence, susceptible to oxidative damage.

Key Words: oxidation • oxidative burst • selenoprotein • phagocytosis • eosinophils • inflammation






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