Journal of Leukocyte Biology
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Originally published online as doi:10.1189/jlb.0307158 on September 12, 2007

Published online before print September 12, 2007
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(Journal of Leukocyte Biology. 2007;82:1592-1604.)
© 2007 by Society for Leukocyte Biology

Transcriptional profiling of human monocytes reveals complex changes in the expression pattern of inflammation-related genes in response to the annexin A1-derived peptide Ac1-25

Carsten Lange, Diane J. Starrett, Julia Goetsch, Volker Gerke and Ursula Rescher1

Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation, and Interdisciplinary Clinical Research Centre, University of Muenster, Muenster, Germany

1Correspondence: Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation, Von-Esmarch-Str. 56, 48149 Muenster, Germany. E-mail: rescher{at}uni-muenster.de

Annexin A1 is a glucocorticoid-regulated, anti-inflammatory protein, which plays an important role as an endogenous regulator of the inflammatory response. Many of these anti-inflammatory properties are retained in the N-terminal annexin A1 peptide Ac1-25, which is released from the full-length protein by a neutrophil elastase. To elucidate whether the anti-inflammatory activity of the bioactive peptide is solely a result of immediate post-translational effects, which include the shedding of L-selectin or also involve transcriptional changes affecting leukocyte function, we recorded global gene expression changes in human monocytes stimulated with exogenously applied Ac1-25. Applying stringent selection criteria, we show that ~100 genes are up-regulated, and ~230 are down-regulated by a factor of at least two in the Ac1-25-treated monocytes. It is important that the profiling reveals that Ac1-25 induces an anti-inflammatory phenotype by down-regulating proinflammatory and up-regulating anti-inflammatory mediators. These effects, elicited by exogenously applied Ac1-25, depend, to different extents, on ERK1/2 and p38 signaling pathways. This identifies the annexin A1 N-terminal peptide as a stimulus, eliciting not only short-term, post-translational effects in human monocytes but also transcriptional changes, defining a more anti-inflammatory profile.

Key Words: gene regulation • anti-inflammation • endogenous mediator • IL-1Ra • CCR2




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V. Goebeler, M. Poeter, D. Zeuschner, V. Gerke, and U. Rescher
Annexin A8 Regulates Late Endosome Organization and Function
Mol. Biol. Cell, December 1, 2008; 19(12): 5267 - 5278.
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