Published online before print August 7, 2007

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(Journal of Leukocyte Biology. 2007;82:1143-1146.)
© 2007 by Society for Leukocyte Biology

Human immature monocyte-derived dendritic cells produce and secrete -defensins 1–3

Marta Rodríguez-García^*,1, Harold Oliva^*,,1, Núria Climent^*,, Felipe García^,, José M. Gatell^, and Teresa Gallart^*,,2

^* Service of Immunology,
Institut d’Investigacions Biomediques August Pi i Sunyer (IDIBAPS)-AIDS Research Group, and
Service Infectious Diseases and AIDS Unit, Hospital Clínic Universitari de Barcelona, Catalonian Center for HIV Vaccines (HIVACAT), School of Medicine, University of Barcelona, Barcelona, Spain

² Correspondence: Service of Immunology, Hospital Clínic Universitari de Barcelona, Villarroel 170, 08036 Barcelona, Spain. E-mail: tgallart{at}clinic.ub.es

ABSTRACT

Defensins are effector molecules of the innate immunity with a broad antimicrobial spectrum, including HIV. They also link innate and adaptive immunity, displaying chemotactic activity for monocytes, T cells, and dendritic cells (DCs). -Defensins 1–3 are mainly produced by neutrophils, but their production by other leukocyte subsets has also been reported. Herein, we studied whether monocyte-derived DCs (MDDCs), which are regarded as a model for myeloid DCs, produce -defensins 1–3. We found that immature MDDCs (imMDDCs) produce -defensins 1–3 mRNA, but this production is undetectable or barely detectable following 48 h of maturation with the proinflammatory cytokine cocktail (IL-1β+IL-6+TNF-) or LPS. It is surprising that -defensins 1–3 production was up-regulated when exposed to each one of the proinflammatory cytokines alone, especially IL-1β. -Defensins 1–3 produced by imMDDCs were mainly secreted peptides. Production and secretion of -defensins 1–3 by imMDDCs can have biological relevance for the antigen processing of pathogens and can contribute to understanding differences in susceptibility to infections, an issue of special interest in the field of HIV infection.

Key Words: human neutrophil peptides 1–3 • real-time PCR • innate immunity

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