Journal of Leukocyte Biology Biosymposia, Inc.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published online as doi:10.1189/jlb.0307140 on July 3, 2007

Published online before print July 3, 2007
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jlb.0307140v1
82/4/887    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rasmusson, I.
Right arrow Articles by Levitsky, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rasmusson, I.
Right arrow Articles by Levitsky, V.
(Journal of Leukocyte Biology. 2007;82:887-893.)
© 2007 by Society for Leukocyte Biology

Mesenchymal stem cells fail to trigger effector functions of cytotoxic T lymphocytes

Ida Rasmusson*,1,2, Michael Uhlin{dagger},1, Katarina Le Blanc*,{dagger} and Victor Levitsky{dagger}

* Division of Clinical Immunology and
{dagger} Microbiology and Tumor Biology Center, and
{dagger} Hematology Center and Division of Clinical Immunology, Karolinska Institutet, Stockholm, Sweden

2 Correspondence: Division of Clinical Immunology, F79, Karolinska University Hospital, SE-141 86 Stockholm, Sweden. E-mail: Ida.Rasmusson{at}ki.se

Mesenchymal stem cells (MSCs), isolated from adult human bone marrow, have immunomodulatory properties. The functional outcomes of MSCs–CTL interactions remain poorly characterized. In this study, we demonstrate that MSCs remain resistant to CTL lysis, even after pulsing with the specific synthetic peptide at high concentrations, in spite of surface expression of the relevant MHC class I allele. MSCs were also much less sensitive to lysis by an allo-specific CTL clone as compared with HLA-matched lymphoblastoid cell lines. MSCs induced CD25 up-regulation, albeit at relatively low levels, and were unable to induce CD3 or CD8 down-regulation at the surface of CTLs. MSCs also failed to induce IFN-{gamma} and TNF-{alpha} production by the CTLs. Furthermore, peptide-pulsed MSCs were inefficient in stimulating tyrosine phosphorylation in specific CTLs. Our results demonstrate that MSCs induce only an abortive activation program in fully differentiated, effector CTLs, which does not involve activation of major CTL effector functions. These data may have important implications for the development of therapeutic strategies based on administration of in vitro-expanded MSCs.

Key Words: marrow stromal cells • effector CD8 T cells • activation • EBV • alloantigen




This article has been cited by other articles:


Home page
 BloodHome page
H. Karlsson, S. Samarasinghe, L. M. Ball, B. Sundberg, A. C. Lankester, F. Dazzi, M. Uzunel, K. Rao, P. Veys, K. Le Blanc, et al.
Mesenchymal stem cells exert differential effects on alloantigen and virus-specific T-cell responses
Blood, August 1, 2008; 112(3): 532 - 541.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
F. Morandi, L. Raffaghello, G. Bianchi, F. Meloni, A. Salis, E. Millo, S. Ferrone, V. Barnaba, and V. Pistoia
Immunogenicity of Human Mesenchymal Stem Cells in HLA-Class I-Restricted T-Cell Responses Against Viral or Tumor-Associated Antigens
Stem Cells, May 1, 2008; 26(5): 1275 - 1287.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 by the Society for Leukocyte Biology.