Pepro Tech
Originally published online as doi:10.1189/jlb.1006598 on May 23, 2007

Published online before print May 23, 2007
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
jlb.1006598v1
82/2/327    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nishioka, T.
Right arrow Articles by Sugawara, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nishioka, T.
Right arrow Articles by Sugawara, S.
(Journal of Leukocyte Biology. 2007;82:327-334.)
© 2007 by Society for Leukocyte Biology

Induction of serum IL-18 with Propionibacterium acnes and lipopolysaccharide in phagocytic macrophage-inactivated mice

Takashi Nishioka*,{dagger}, Toshinobu Kuroishi*, Yumiko Sugawara{dagger}, Zhiqian Yu{dagger}, Takashi Sasano{dagger}, Yasuo Endo* and Shunji Sugawara*,1

* Division of Oral Immunology, Department of Oral Biology, and
{dagger} Division of Oral Diagnosis, Department of Oral Medicine and Surgery, Tohoku University Graduate School of Dentistry, Sendai, Japan

1 Correspondence: Division of Oral Immunology, Department of Oral Biology, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan. E-mail: sugawars{at}mail.tains.tohoku.ac.jp

IL-18, an important regulator of immune responses, is expressed in activated macrophages and also in nonimmune cells, such as keratinocytes and epithelial cells. Increased levels of serum IL-18 are reported in patients with a wide variety of diseases, but it is unclear which type of cell is the major source of serum IL-18. Here, we showed that the administration of liposomes encapsulating clodronate (Clo-lip) in mice selectively depleted F4/80+ phagocytic macrophages in the liver and spleen. Serum levels of mature IL-18 with 18 kDa were increased markedly in mice treated with Propionibacterium acnes and LPS, whereas administration of Clo-lip and gadolinium chloride, another widely used macrophage inactivator, showed no obvious effect on serum IL-18 levels, which were marginal in the liver, lung, and spleen and more pronounced in the intestines, especially in the duodenum. Treatment with P. acnes alone induced IL-18 more than twofold in each organ, and P. acnes and LPS induced a marked increase in IL-18 levels in the liver and spleen but decreased in the intestines. The administration of Clo-lip showed only a marginal effect on the IL-18 levels in these organs. Furthermore, serum levels of liver enzymes and TNF-{alpha} and liver injury (necrotic change and granuloma formation) induced by P. acnes and LPS were reduced moderately by Clo-lip. These results suggest that phagocytic macrophages do not actively contribute to the induction of serum IL-18 and liver injury in mice treated with P. acnes and LPS.

Key Words: cytokine • inflammation • macrophages • mucosal cells




This article has been cited by other articles:


Home page
 J. Immunol.Home page
A. Sakai, Y. Sugawara, T. Kuroishi, T. Sasano, and S. Sugawara
Identification of IL-18 and Th17 Cells in Salivary Glands of Patients with Sjogren's Syndrome, and Amplification of IL-17-Mediated Secretion of Inflammatory Cytokines from Salivary Gland Cells by IL-18
J. Immunol., August 15, 2008; 181(4): 2898 - 2906.
[Abstract] [Full Text] [PDF]