Accuri C6 Flow Cytometer System
Originally published online as doi:10.1189/jlb.0207089 on April 5, 2007

Published online before print April 5, 2007
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(Journal of Leukocyte Biology. 2007;82:85-92.)
© 2007 by Society for Leukocyte Biology

Regulatory function of CD4+CD25+ T cells from Class II MHC-deficient mice in contact hypersensitivity responses

Danielle D. Kish*,1, Anton V. Gorbachev* and Robert L. Fairchild*,{dagger},{ddagger}

* Department of Immunology and
{dagger} Urological Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA; and
{ddagger} Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA

1 Correspondence: NB3-30, Department of Immunology, Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, OH 44195-0001, USA. E-mail: kishd{at}ccf.org

Contact hypersensitivity (CHS) is a CD8+ T cell-mediated, inflammatory response to hapten sensitization and challenge of the skin. During sensitization, the magnitude and duration of hapten-specific CD8+ T cell expansion in the skin-draining lymph nodes (LN) are restricted by CD4+CD25+ T regulatory cells (Treg). The regulation of hapten-specific CD8+ T cell priming in Class II MHC-deficient (MHC–/–) mice was investigated. Although hapten-specific CD8+ T cell priming and CHS responses were elevated in Class II MHC–/– versus wild-type mice, presensitization depletion of CD4+ or CD25+ cells in Class II MHC–/– mice further increased CD8+ T cell priming and the elicited CHS response. Flow cytometry analyses of LN cells from Class II MHC–/– mice revealed a population of CD4+ T cells with a majority expressing CD25. Forkhead box p3 mRNA was expressed in LN cells from Class II MHC–/– and was reduced to background levels by depletion of CD4+ or CD25+ cells. Isolated CD4+CD25+ T cells from wild-type and Class II MHC–/– mice limited in vitro proliferation of alloantigen- and hapten-specific T cells to antigen-presenting stimulator cells. These results identify functional CD4+CD25+ Treg in Class II MHC–/– mice, which restrict hapten-specific CD8+ T cell priming and the magnitude of CHS responses.

Key Words: suppression • Treg • MHC–/–




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