Pepro Tech
Originally published online as doi:10.1189/jlb.0406267 on April 30, 2007

Published online before print April 30, 2007
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
jlb.0406267v1
82/1/133    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kwan, W.-H.
Right arrow Articles by Mueller, C. G. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kwan, W.-H.
Right arrow Articles by Mueller, C. G. F.
(Journal of Leukocyte Biology. 2007;82:133-141.)
© 2007 by Society for Leukocyte Biology

LPS induces rapid IL-10 release by M-CSF-conditioned tolerogenic dendritic cell precursors

Wing-Hong Kwan1, Charlotte Boix, Nicolas Gougelet, Wolf H. Fridman and Chris G. F. Mueller2

INSERM UMRS 872, Université Pierre et Marie Curie-Paris 6, Université René Descartes-Paris 5, Centre de Recherches Biomédicales des Cordeliers, Paris, France

1 Correspondence: INSERM UMRS 872, Université Pierre et Marie Curie-Paris 6, Université René Descartes-Paris 5, Centre de Recherches Biomédicales des Cordeliers, 15, Rue de l’Ecole de Médecine, 75006 Paris, France. E-mail: wing-hong.kwan{at}u255.bhdc.jussieu.fr

Dendritic cells (DC) obtained by culturing myeloid precursors in GM-CSF undergo maturation and induce an efficient T cell response when stimulated with microbial products. DC precursors themselves also recognize microbial products, and it remains unclear how these stimulated DC precursors modulate the immune response. We show here that M-CSF-conditioned human DC precursors responded to LPS, Mycobacteria bovis, and inflammatory cytokines by a rapid and robust production of IL-10, largely superior to that observed with immature DC or monocytes. The endogenous IL-10 restrained the DC precursors from converting into professional APC, as blocking the IL-10 receptor in the presence of LPS resulted in the formation of efficient T cell stimulators. LPS stimulation concomitant with DC differentiation gave rise to immature DC, which were tolerant to a secondary LPS exposure. Furthermore, the LPS-activated DC precursors reduced bystander DC maturation and anti-CD3/CD28-triggered T cell activation. These data suggest that when exposed to inflammatory or microbial signals, M-CSF-conditioned DC precursors can participate in the modulation of inflammation and immune response by rapid release of IL-10.

Key Words: growth factor • cytokine • differentiation • activation • tolerance




This article has been cited by other articles:


Home page
 Int ImmunolHome page
B. B. Ganesh, D. M. Cheatem, J. R. Sheng, C. Vasu, and B. S. Prabhakar
GM-CSF-induced CD11c+CD8a--dendritic cells facilitate Foxp3+ and IL-10+ regulatory T cell expansion resulting in suppression of autoimmune thyroiditis
Int. Immunol., March 1, 2009; 21(3): 269 - 282.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
P. P. Sarangi, B. Kim, E. Kurt-Jones, and B. T. Rouse
Innate Recognition Network Driving Herpes Simplex Virus-Induced Corneal Immunopathology: Role of the Toll Pathway in Early Inflammatory Events in Stromal Keratitis
J. Virol., October 15, 2007; 81(20): 11128 - 11138.
[Abstract] [Full Text] [PDF]