Journal of Leukocyte Biology Myeloid cells, immune suppression, tumor immunology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published online as doi:10.1189/jlb.1006615 on January 31, 2007

Published online before print January 31, 2007
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jlb.1006615v1
81/4/952    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Noone, C.
Right arrow Articles by Johnson, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Noone, C.
Right arrow Articles by Johnson, P.
(Journal of Leukocyte Biology. 2007;81:952-956.)
© 2007 by Society for Leukocyte Biology

Artificially generated dendritic cells misdirect antiviral immune responses

Cariosa Noone*, Ellen Manahan*, Robert Newman{dagger} and Patricia Johnson*,1

* Viral Immunology Group, School of Biotechnology, Dublin City University, Dublin, Ireland; and
{dagger} National Institute of Biological Standards and Controls, Herts, UK

1 Correspondence: Viral Immunology Group, School of Biotechnology, Dublin City University, Dublin 9, Ireland. E-mail: patricia.johnson{at}dcu.ie

Dendritic cells (DCs) are critical to the outcome of many viral infections. Questions still remain as to the relevance of artificially generated DCs in models of in vivo immune responses. We compared different DC generation pathways, in terms of phenotypic expression, cytokine production, apoptosis, and T cell proliferation, following viral infection. Direct viral infection of monocytes or monocytes cultured with supernatants from virally infected lung epithelial cells (A549 DCs) induce distinct DC subsets compared with viral infection of artificially generated IL-4 DCs and IFN-DCs. These virally infected DC subsets stimulated different cytokine secretion profiles and displayed contrasting sensitivities to viral-induced apoptosis. It is most interesting that we observed marked differences in the proliferation of purified CD3+ T cells from the virally infected DC subsets. In conclusion, artificially generated DCs skew immune responses to viral infections, and direct viral infection of monocytes and DCs, generated from monocytes cultured with supernatants from infected epithelial cells, appears to be a more relevant pathway of producing DCs, which mimic those generated in vivo.

Key Words: monocytes • T cells • viral infections




This article has been cited by other articles:


Home page
 J. Virol.Home page
C. M. Noone, E. Paget, E. A. Lewis, M. R. Loetscher, R. W. Newman, and P. A. Johnson
Natural Killer Cells Regulate T-Cell Proliferation during Human Parainfluenza Virus Type 3 Infection
J. Virol., September 15, 2008; 82(18): 9299 - 9302.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 by the Society for Leukocyte Biology.