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Published online before print October 31, 2006

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(Journal of Leukocyte Biology. 2007;81:458-464.)
© 2007 by Society for Leukocyte Biology

Proteinase 3 and CD177 are expressed on the plasma membrane of the same subset of neutrophils

Susanne Bauer^*, Mohamed Abdgawad, Lena Gunnarsson, Mårten Segelmark, Hans Tapper^* and Thomas Hellmark^,1

^* Departments of Clinical Sciences, Section for Clinical and Experimental Infection Medicine, and
Nephrology, Clinical Sciences in Lund, Lund University, Lund, Sweden

¹ Correspondence: BMC-C14, Dept. of Nephrology, Clinical Sciences in Lund, Lund University, 221 84 Lund, Sweden. E-mail: thomas.hellmark{at}med.lu.se

Proteinase 3 (PR3) is found in granules of all neutrophils but also on the plasma membrane of a subset of neutrophils (mPR3). CD177, another neutrophil protein, also displays a bimodal surface expression. In this study, we have investigated the coexpression of these two molecules, as well as the effect of cell activation on their surface expression. We can show that CD177 is expressed on the same subset of neutrophils as mPR3. Experiments show that the expression of mPR3 and CD177 on the plasma membrane is increased or decreased in parallel during cell stimulation or spontaneous apoptosis. Furthermore, we observed a rapid internalization and recirculation of mPR3 and plasma membrane CD177, where all mPR3 is replaced within 30 min. Our findings suggest that the PR3 found on the plasma membrane has its origin in the same intracellular storage as CD177, i.e., secondary granules and secretory vesicles and not primary granules. PR3- and CD177-expressing neutrophils constitute a subpopulation of neutrophils with an unknown role in the innate immune system, which may play an important role in diseases such as Wegener’s granulomatosis and polycythemia vera.

Key Words: trafficking • colocalization • serine proteases • Wegener’s granulomatosis • polycythemia vera

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