| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Published online before print October 11, 2006
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* Clinical Immunology Unit and
Chromatin Dynamics Unit, H San Raffaele Scientific Institute and Università Vita-Salute San Raffaele, Milan, Italy
1Correspondence: Cancer Immunotherapy and Gene Therapy Program, Clinical Immunology Unit, H. San Raffaele Scientific Institute, via Olgettina 58, Milano 20132, Italy. E-mail: rovere.patrizia{at}hsr.it
ABSTRACT
Chemokines regulate the migration and the maturation of dendritic cells (DC) licensed by microbial constituents. We have recently found that the function of DC, including their ability to activate naïve, allogeneic CD4+ T cells, requires the autocrine/paracrine release of the nuclear protein high mobility group box 1 (HMGB1). We show here that human myeloid DC, which rapidly secrete upon maturation induction their own HMGB1, remodel their actin-based cytoskeleton, up-regulate the CCR7 and the CXCR4 chemokine receptors, and acquire the ability to migrate in response to chemokine receptor ligands. The events are apparently causally related: DC challenged with LPS in the presence of HMGB1-specific antibodies fail to up-regulate the expression of the CCR7 and CXCR4 receptors and to rearrange actin-rich structures. Moreover, DC matured in the presence of anti-HMGB1 antibodies fail to migrate in response to the CCR7 ligand CCL19 and to the CXCR4 ligand CXCL12. The blockade of receptor for advanced glycation end products (RAGE), the best-characterized membrane receptor for HMGB1, impinges as well on the up-regulation of chemokine receptors and on responsiveness to CCL19 and CXCL12. Our data suggest that the autocrine/paracrine release of HMGB1 and the integrity of the HMGB1/RAGE pathway are required for the migratory function of DC.
Key Words: cell trafficking • cell death
This article has been cited by other articles:
|
|
 |
|
  J. H. H. Williams and H. E. Ireland Sensing danger--Hsp72 and HMGB1 as candidate signals J. Leukoc. Biol., March 1, 2008; 83(3): 489 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Manfredi, A. Capobianco, A. Esposito, F. De Cobelli, T. Canu, A. Monno, A. Raucci, F. Sanvito, C. Doglioni, P. P. Nawroth, et al. Maturing Dendritic Cells Depend on RAGE for In Vivo Homing to Lymph Nodes J. Immunol., February 15, 2008; 180(4): 2270 - 2275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-H. Yen, T. Khayrullina, and D. Ganea PGE2-induced metalloproteinase-9 is essential for dendritic cell migration Blood, January 1, 2008; 111(1): 260 - 270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Moser, D. D. Desai, M. P. Downie, Y. Chen, S. F. Yan, K. Herold, A. M. Schmidt, and R. Clynes Receptor for Advanced Glycation End Products Expression on T Cells Contributes to Antigen-Specific Cellular Expansion In Vivo J. Immunol., December 15, 2007; 179(12): 8051 - 8058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Herold, B. Moser, Y. Chen, S. Zeng, S. F. Yan, R. Ramasamy, J. Emond, R. Clynes, and A. M. Schmidt Receptor for advanced glycation end products (RAGE) in a dash to the rescue: inflammatory signals gone awry in the primal response to stress J. Leukoc. Biol., August 1, 2007; 82(2): 204 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Bianchi DAMPs, PAMPs and alarmins: all we need to know about danger J. Leukoc. Biol., January 1, 2007; 81(1): 1 - 5. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
