Journal of Leukocyte Biology eBioscience full spectrum cell analysis
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published online as doi:10.1189/jlb.0306200 on September 15, 2006

Published online before print September 15, 2006
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jlb.0306200v1
81/1/49    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rouhiainen, A.
Right arrow Articles by Rauvala, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rouhiainen, A.
Right arrow Articles by Rauvala, H.
Related Collections
Right arrowRelated Article
(Journal of Leukocyte Biology. 2007;81:49-58.)
© 2007 by Society for Leukocyte Biology

Analysis of proinflammatory activity of highly purified eukaryotic recombinant HMGB1 (amphoterin)

Ari Rouhiainen1, Sarka Tumova, Leena Valmu, Nisse Kalkkinen and Heikki Rauvala

Neuroscience Center, and Institute of Biotechnology, University of Helsinki, Helsinki, Finland

1Correspondence: Neuroscience Center, Viikinkaari 4, PL 56, University of Helsinki, Helsinki 00014, Finland. E-mail: ari.rouhiainen{at}helsinki.fi

ABSTRACT

HMGB1 (amphoterin) is a 30-kDa heparin-binding protein that mediates transendothelial migration of monocytes and has proinflammatory cytokine-like activities. In this study, we have investigated proinflammatory activities of both highly purified eukaryotic HMGB1 and bacterially produced recombinant HMGB1 proteins. Mass analyses revealed that recombinant eukaryotic HMGB1 has an intrachain disulphide bond. In mass analysis of tissue-derived HMGB1, two forms were detected: the carboxyl terminal glutamic acid residue lacking form and a full-length form. Cell culture studies indicated that both eukaryotic and bacterial HMGB1 proteins induce TNF-{alpha} secretion and nitric oxide release from mononuclear cells. Affinity chromatography analysis revealed that HMGB1 binds tightly to proinflammatory bacterial substances. A soluble proinflammatory substance was separated from the bacterial recombinant HMGB1 by chloroform-methanol treatment. HMGB1 interacted with phosphatidylserine in both solid-phase binding and cell culture assays, suggesting that HMGB1 may regulate phosphatidylserine-dependent immune reactions. In conclusion, HMGB1 polypeptide has a weak proinflammatory activity by itself, and it binds to bacterial substances, including lipids, that may strengthen its effects.

Key Words: inflammation • nitric oxide • phosphatidylserine • RAGE • TNF-{alpha}


Related Article

Interview with Dr. Heikki Rauvala regarding Pivotal Advance: Analysis of proinflammatory activity of highly purified eukaryotic recombinant HMGB1 (amphoterin)
Marco E. Bianchi
J. Leukoc. Biol. 2007 81: 46-48. [Full Text] [PDF]



This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
R. K. Aneja, A. Tsung, H. Sjodin, J. V. Gefter, R. L. Delude, T. R. Billiar, and M. P. Fink
Preconditioning with high mobility group box 1 (HMGB1) induces lipopolysaccharide (LPS) tolerance
J. Leukoc. Biol., November 1, 2008; 84(5): 1326 - 1334.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. M. Rowe, P. L. Jackson, G. Liu, M. Hardison, A. Livraghi, G. M. Solomon, D. B. McQuaid, B. D. Noerager, A. Gaggar, J. P. Clancy, et al.
Potential Role of High-Mobility Group Box 1 in Cystic Fibrosis Airway Disease
Am. J. Respir. Crit. Care Med., October 15, 2008; 178(8): 822 - 831.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. Liu, J. Wang, Y.-J. Park, Y. Tsuruta, E. F. Lorne, X. Zhao, and E. Abraham
High Mobility Group Protein-1 Inhibits Phagocytosis of Apoptotic Neutrophils through Binding to Phosphatidylserine
J. Immunol., September 15, 2008; 181(6): 4240 - 4246.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
A. Zicari, C. Centonze, M. Realacci, B. Buchetti, A. Pietropolli, and C. Ticconi
Estradiol 17-{beta} and Progesterone Modulate Inducible Nitric Oxide Synthase and High Mobility Group Box 1 Expression in Human Endometrium
Reproductive Sciences, July 1, 2008; 15(6): 559 - 566.
[Abstract] [PDF]

Home page
 J. Immunol.Home page
J. H. Youn, Y. J. Oh, E. S. Kim, J. E. Choi, and J.-S. Shin
High Mobility Group Box 1 Protein Binding to Lipopolysaccharide Facilitates Transfer of Lipopolysaccharide to CD14 and Enhances Lipopolysaccharide-Mediated TNF-{alpha} Production in Human Monocytes
J. Immunol., April 1, 2008; 180(7): 5067 - 5074.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
J. H. H. Williams and H. E. Ireland
Sensing danger--Hsp72 and HMGB1 as candidate signals
J. Leukoc. Biol., March 1, 2008; 83(3): 489 - 492.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Sha, J. Zmijewski, Z. Xu, and E. Abraham
HMGB1 Develops Enhanced Proinflammatory Activity by Binding to Cytokines
J. Immunol., February 15, 2008; 180(4): 2531 - 2537.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. M. Levy, K. P. Mollen, J. M. Prince, D. J. Kaczorowski, R. Vallabhaneni, S. Liu, K. J. Tracey, M. T. Lotze, D. J. Hackam, M. P. Fink, et al.
Systemic inflammation and remote organ injury following trauma require HMGB1
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1538 - R1544.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
D. S. Pisetsky
The Role of Nuclear Macromolecules in Innate Immunity
Proceedings of the ATS, July 1, 2007; 4(3): 258 - 262.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 by the Society for Leukocyte Biology.