Journal of Leukocyte Biology eBioscience full spectrum cell analysis
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Originally published online as doi:10.1189/jlb.0206124 on October 17, 2006

Published online before print October 17, 2006
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(Journal of Leukocyte Biology. 2007;81:328-335.)
© 2007 by Society for Leukocyte Biology

cDNA microarray analysis reveals fundamental differences in the expression profiles of primary human monocytes, monocyte-derived macrophages, and alveolar macrophages

Jiangning Li*,1, David K. Pritchard*,1,2, Xi Wang*,3, David R. Park{dagger}, Roger E. Bumgarner{ddagger}, Stephen M. Schwartz* and W. Conrad Liles*,{dagger},4

Departments of
* Pathology,
{dagger} Medicine, and
{ddagger} Microbiology, University of Washington, Seattle, Washington, USA

2Correspondence: UW Medicine–South Lake Union, 815 Mercer Street, Seattle, WA 98109-4714, USA. E-mail: dpritch{at}u.washington.edu

We report the systematic use of large-scale cDNA microarrays to study the gene expression profiles of primary human peripheral blood monocytes (MONO) in comparison with in vitro-differentiated, M-CSF-induced MONO-derived macrophages (MAC) and primary human alveolar MAC (AM), obtained by bronchoalveolar lavage from the lungs of normal volunteers. These studies revealed large-scale differences in the gene expression profile between both MAC types (MAC and AM) and MONO. In addition, large differences were observed in the gene expression profiles of the two MAC types. Specifically, 21% of genes on the array (2904 out of 13,582) were differentially expressed between AM and MONO, and 2229 out of 13,583 probes were differentially expressed between MAC and AM. Our expression data show remarkable differences in gene expression between different MAC subpopulations and emphasize the heterogeneity of different MAC populations. This study underscores the need to scrutinize models of MAC biology for relevance to specific disease processes.

Key Words: differentiation • heterogeneity • gene expression




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