HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Published online before print September 7, 2006

This Article Full Text Full Text (PDF) All Versions of this Article: jlb.0206111v1 80/6/1337    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Santegoets, S. J. A. M. Articles by de Gruijl, T. D. Search for Related Content PubMed PubMed Citation Articles by Santegoets, S. J. A. M. Articles by de Gruijl, T. D.

(Journal of Leukocyte Biology. 2006;80:1337-1344.)
© 2006 by Society for Leukocyte Biology

A CD34⁺ human cell line model of myeloid dendritic cell differentiation: evidence for a CD14⁺CD11b⁺ Langerhans cell precursor

Saskia J. A. M. Santegoets^*,1, Allan J. Masterson^,1, Pieter C. van der Sluis^*, Sinéad M. Lougheed, Donna M. Fluitsma, Alfons J. M. van den Eertwegh, Herbert M. Pinedo, Rik J. Scheper^*,2 and Tanja D. de Gruijl

^* Departments of Pathology,
Medical Oncology, and
Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands

² Correspondence: Department of Pathology, VU University Medical Center, De Boelelaan 1117, Amsterdam 1081HV, The Netherlands. E-mail: rj.scheper{at}vumc.nl

The study of early events in dendritic cell (DC) differentiation is hampered by the lack of homogeneous primary cell systems that allow the study of cytokine-driven, transitional DC differentiation steps. The CD34⁺ acute myeloid leukemia cell line MUTZ-3 displays a unique ability to differentiate into interstitial DC (IDC) and Langerhans cells (LC) in a cytokine-dependent manner. Phenotypic characterization revealed MUTZ-3 to consist of three distinct subpopulations. Small CD34⁺CD14^–CD11b^– progenitors constitute the proliferative compartment of the cell line with the ability to differentiate through a CD34^–CD14^–CD11b⁺ stage to ultimately give rise to a morphologically large, nonproliferating CD14⁺CD11b^hi progeny. These CD14⁺CD11b^hi cells were identified as common, immediate myeloid DC precursors with the ability to differentiate into LC and IDC, exhibiting characteristic and mutually exclusive expression of Langerin and DC-specific ICAM-grabbing nonintegrin, respectively. The identity of the MUTZ-3-derived LC subset was confirmed further by the presence of Birbeck granules. We conclude that the MUTZ-3 cell line provides a ready and continuous supply of common myeloid precursors, which should facilitate further study of the ontogeny of myeloid DC lineages.

Key Words: MUTZ-3 • progenitor • acute myeloid leukemia • cytokines

This article has been cited by other articles:

				M. Moreno, J. W. Molling, S. von Mensdorff-Pouilly, R. H. M. Verheijen, E. Hooijberg, D. Kramer, A. W. Reurs, A. J. M. van den Eertwegh, B. M. E. von Blomberg, R. J. Scheper, et al. IFN-{gamma}-Producing Human Invariant NKT Cells Promote Tumor-Associated Antigen-Specific Cytotoxic T Cell Responses J. Immunol., August 15, 2008; 181(4): 2446 - 2454. [Abstract] [Full Text] [PDF]

				S. J. A. M. Santegoets, H. J. Bontkes, A. G. M. Stam, F. Bhoelan, J. J. Ruizendaal, A. J. M. van den Eertwegh, E. Hooijberg, R. J. Scheper, and T. D. de Gruijl Inducing Antitumor T Cell Immunity: Comparative Functional Analysis of Interstitial Versus Langerhans Dendritic Cells in a Human Cell Line Model J. Immunol., April 1, 2008; 180(7): 4540 - 4549. [Abstract] [Full Text] [PDF]