Comparative gene expression by WC1+ {gamma}{delta} and CD4+ {alpha}{beta} T lymphocytes, which respond to Anaplasma marginale, demonstrates higher expression of chemokines and other myeloid cell-associated genes by WC1+ {gamma}{delta} T cells

doi:10.1189/jlb.0506353

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Comparative gene expression by WC1⁺ ${gamma}$ ${delta}$ and CD4⁺ ${alpha}$ ß T lymphocytes, which respond to Anaplasma marginale, demonstrates higher expression of chemokines and other myeloid cell-associated genes by WC1⁺ ${gamma}$ ${delta}$ T cells

Kevin K. Lahmers^*, Jodi F. Hedges, Mark A. Jutila, Mingqi Deng, Mitchell S. Abrahamsen and Wendy C. Brown^*^,1

^* Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington;
Veterinary Molecular Biology Laboratory, Montana State University, Bozeman, Montana; and
Department of Veterinary Pathobiology, University of Minnesota, St. Paul, Minnesota

¹ Correspondence: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040. E-mail: wbrown{at}vetmed.wsu.edu

The functions of ${gamma}$ ${delta}$ T cells are enigmatic, and these cells areoften considered as evolutionary remnants of well-characterized ${alpha}$ ß T cells. However, their conservation throughoutevolution suggests that ${gamma}$ ${delta}$ T cells are biologically unique. Inruminants, ${gamma}$ ${delta}$ T cells expressing the workshop cluster 1 (WC1)scavenger receptor comprise a large proportion of circulatinglymphocytes, suggesting these cells are biologically relevantand functionally different from ${alpha}$ ß T cells. In fact,bovine WC1⁺ ${gamma}$ ${delta}$ T cells can act as APC for ${alpha}$ ß T cells,indicating they may express genes encoding proteins associatedwith innate immunity. The present study was designed to compareimmune function gene expression profiles of clonal populationsof WC1⁺ ${gamma}$ ${delta}$ and CD4⁺ ${alpha}$ ß T cells derived from the sameanimal, which respond to major surface protein 2 (MSP2) of theintraerythrocytic rickettsial pathogen of cattle, Anaplasmamarginale. Gene expression profiles of activated T cell cloneswere compared using a microarray format, and differential geneexpression was confirmed by real-time RT-PCR and protein analyses.We demonstrate that although MSP2-specific ${alpha}$ ß and ${gamma}$ ${delta}$ T cell clones express many of the same genes, ${gamma}$ ${delta}$ T cell clonesexpress high levels of genes associated with myeloid cells,including chemokines CCL2, CXCL1, CXCL2, CXCL6, and surfacereceptors CD68, CD11b, macrophage scavenger receptor 1, macrophagemannose receptor, and galectin-3. It is important that manyof these genes were also expressed at higher levels in polyclonalWC1⁺ ${gamma}$ ${delta}$ T cells when compared with CD4⁺ ${alpha}$ ß T cells selectedfrom peripheral blood.

Key Words: MSP2 • macrophage scavenger receptor 1 • macrophage mannose receptor • and galectin-3

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Comparative gene expression by WC1+ and CD4+ ß T lymphocytes, which respond to Anaplasma marginale, demonstrates higher expression of chemokines and other myeloid cell-associated genes by WC1+ T cells

Comparative gene expression by WC1⁺ ${gamma}$ ${delta}$ and CD4⁺ ${alpha}$ ß T lymphocytes, which respond to Anaplasma marginale, demonstrates higher expression of chemokines and other myeloid cell-associated genes by WC1⁺ ${gamma}$ ${delta}$ T cells