Journal of Leukocyte Biology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published online as doi:10.1189/jlb.0106015 on July 24, 2006

Published online before print July 24, 2006
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jlb.0106015v1
80/4/816    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Meindl, S.
Right arrow Articles by Elbe-Bürger, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Meindl, S.
Right arrow Articles by Elbe-Bürger, A.
(Journal of Leukocyte Biology. 2006;80:816-826.)
© 2006 by Society for Leukocyte Biology

Characterization, isolation, and differentiation of murine skin cells expressing hematopoietic stem cell markers

Simone Meindl*, Uwe Schmidt{dagger}, Christine Vaculik* and Adelheid Elbe-Bürger*,1

* Department of Dermatology, Division of Immunology, Allergy and Infectious Diseases, and
{dagger} Institute of Immunology, Vienna Competence Center, Medical University of Vienna, Vienna, Austria

1 Correspondence: Department of Dermatology, Division of Immunology, Allergy and Infectious Diseases, Vienna Competence Center, Medical University of Vienna, Lazarettgasse 19, Vienna 1090 , Austria. E-mail: adelheid.elbe-buerger{at}meduniwien.ac.at

As the phenotype of adult dermal stem cells is still elusive, and the hematopoietic stem cell is one of the best-characterized stem cells in the body, we tested dermal cell suspensions, sections, and wholemounts in newborn and adult mice for hematopoietic stem cell marker expression. Phenotypic analysis revealed that a small population of CD45+ cells and a large population of CD45– cells expressed CD34, CD117, and stem cell antigen-1 molecules. When cultivated in selected media supplemented with hematopoietic cytokines, total dermal cells, lineage–, and/or highly enriched phenotypically defined cell subsets produced hematopoietic and nonhematopoietic colonies. When injected into lethally irradiated recipient mice, a small percentage of newborn dermal cells was able to migrate into hematopoietic tissues and the skin and survived through the 11-month monitoring period. Our ability to isolate a candidate autologous stem cell pool will make these cells ideal vehicles for genetic manipulation and gene therapy.

Key Words: dermis • mouse • single cell suspension • semisolid medium • hematopoietic cytokines






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the Society for Leukocyte Biology.