Accuri C6 Flow Cytometer System
Originally published online as doi:10.1189/jlb.0306176 on August 2, 2006

Published online before print August 2, 2006
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(Journal of Leukocyte Biology. 2006;80:797-801.)
© 2006 by Society for Leukocyte Biology

T cells traffic from brain to cervical lymph nodes via the cribroid plate and the nasal mucosa

Jana Goldmann*,{dagger}, Erik Kwidzinski*,{dagger}, Christine Brandt*, Jacqueline Mahlo*,{dagger}, Daniel Richter* and Ingo Bechmann*,{dagger},1

* Center for Anatomy, Institute of Cell Biology and Neurobiology, Department of Experimental Neuroimmunology, Charité, Universitätsmedizin Berlin, Berlin, Germany; and
{dagger} Institute of Clinical Neuroanatomy, J. W. Goethe-University, Frankfurt/Main, Germany

1 Correspondence: Institute of Clinical Neuroanatomy, J. W. Goethe-University, Theodor-Stern-Kai 7, Frankfurt/Main 60 590, Germany, E-mail: Bechmann{at}med.uni-frankfurt.de

ABSTRACT

Although drainage pathways of soluble antigens from brain to cervical lymph nodes have been well established, there is no direct evidence for similar routes of leukocytes leaving the central nervous system. We developed a protocol allowing the cross-sectioning of an entire head-neck preparation while preserving the signal of the GFP. We monitored how GFP-expressing CD4 T lymphocytes injected into the entorhinal cortex after lesion or the lateral ventricle of unlesioned C57/bl6 mice reach cervical lymph nodes. Irrespective of the injection site, we demonstrate their passage through the cribroid plate, appearance in the nasal mucosa, and specific accumulation in one of the cervical lymph nodes.

Key Words: CNS • cell trafficking • immune tolerance