Published online before print June 29, 2006
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,¶,1
* Department of Biology, University of Rome Tor Vergata, Italy;
Institute of Neurobiology and Molecular Medicine, Italian National Council of Research, Rome;
Department of Clinical and Behavioural Neurology, IRCCS Santa Lucia Foundation, Rome, Italy; and
National Institute for Infectious Disease "L. Spallanzani," IRCCS, Rome, Italy;
¶ Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy; and
|| Centre International "Chantal Biya" Yaoundè, Cameroun
1 Correspondence: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale regina Elena 299, 00161 Rome, Italy. E-mail: vendetti{at}iss.it
Interleukin (IL)-2 plays an important role in the control of the immune responses, and it is released in a variety of tissues in response to inflammatory stimuli. As monocytes and mature dendritic cells (DCs) express CD25, the high-affinity subunit of IL-2 receptor, we examined the effect of exogenous IL-2 on the in vitro generation and maturation of DCs from monocytes. Human monocyte-derived DCs (MDDCs) were generated by culturing monocytes with granulocyte macrophage-colony stimulating factor (GM-CSF) and IL-4 in the presence or absence of IL-2. The cytokine was added at the beginning and after 5 days of culture. Our findings indicate that IL-2 does induce monocytes to differentiate into DCs with the same morphology and phenotype of that obtained in the presence of GM-CSF and IL-4 alone, but with some distinctive functional properties. DCs differentiated in the presence of IL-2 secreted significantly more IL-1ß, TNF-
, and IL-12 p70 in response to lipopolysaccharide stimulation and induced allogeneic, naïve T cells to release a significantly higher amount of interferon-
if compared with DCs obtained by culturing monocytes with GM-CSF and IL-4. These results indicate unrecognized effects of IL-2 on human MDDCs and suggest that an IL-2-rich environment during differentiation and maturation of DCs can modify their T helper cell-inducing properties.
Key Words: antigen-presenting cell • T cell polarization • cell differentiation
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