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Originally published online as doi:10.1189/jlb.0505280 on October 4, 2005

Published online before print October 4, 2005
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(Journal of Leukocyte Biology. 2005;78:1136-1141.)
© 2005 by Society for Leukocyte Biology

HMGN2: a novel antimicrobial effector molecule of human mononuclear leukocytes?

Yun Feng, Ning Huang, Qi Wu and Boyao Wang1

Research Unit of Infection and Immunity, West China Medical Center, Sichuan University

1Correspondence: Research Unit of Infection and Immunity, West China Medical Center, Sichuan University, Chengdu, Sichuan 610041, PR China. E-mail: wangby{at}mail.sc.cninfo.net

Leukocytes are a central cellular element of innate-immune defense in mammals. In addition to the generation of toxic oxygen radicals and nitric oxide, leukocytes express and secrete a broad array of antimicrobial proteins and peptides. In the study, an antimicrobial polypeptide was isolated and purified from human peripheral blood mononuclear leukocytes in the presence of interleukin (IL)-2. Microsequencing provided that its N-terminal amino sequence was PKRKAEGDAK, which was identical to high mobility group nucleosomal-binding domain 2 (HMGN2). Mass spectrometric value and Western blot also indicated its individual character of HMGN2. The antimicrobial assays showed that the Escherichia coli-based production of HMGN2 had a potent antimicrobial activity against E. coli ML-35p, Pseudomonas aeruginosa ATCC 27853, and to some extent, against Candida albicans ATCC 10231. The HMGN2 {alpha}-helical domain had the same antimicrobial activity as HMGN2. The immunocytochemistry staining, enzyme-linked immunosorbent assay, and Western blot revealed that HMGN2 was present in the cytoplasm of mononuclear leukocytes and released to the extracellular environment when stimulated with IL-2. These results suggest that HMGN2 would be a novel antimicrobial effector molecule of human mononuclear leukocyte.

Key Words: antimicrobial activity • {alpha}-helical domain • subcellular distribution




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[Abstract] [Full Text] [PDF]


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