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Published online before print July 6, 2005

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(Journal of Leukocyte Biology. 2005;78:725-735.)
© 2005 by Society for Leukocyte Biology

CD8⁺ T cells produce IL-2, which is required for CD4⁺CD25⁺ T cell regulation of effector CD8⁺ T cell development for contact hypersensitivity responses

Danielle D. Kish^*,1, Anton V. Gorbachev^* and Robert L. Fairchild^*,

^* Department of Immunology, Cleveland Clinic Foundation, Ohio; and
Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, Ohio

¹Correspondence: NB3-30, Department of Immunology, Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, OH 44195-0001. E-mail: kishd{at}ccf.org

Interleukin (IL)-2 functions to promote, as well as down-regulate, expansion of antigen-reactive CD4⁺ and CD8⁺ T cells, but the role of IL-2 in hapten-specific CD8⁺ T cell priming for contact hypersensitivity (CHS) responses remains untested. Using enzyme-linked immunospot to enumerate numbers of hapten-specific CD4⁺ and CD8⁺ T cells producing IL-2 in hapten-sensitized mice, the number of IL-2-producing CD8⁺ T cells was tenfold that of CD4⁺ T cells. Hapten-primed CD4⁺ T cells produced low amounts of IL-2 during culture with hapten-presenting Langerhans cells, whereas production by hapten-primed CD8⁺ T cells was fivefold greater. CD8⁺ T cells did not express CD25 during hapten priming, but treatment with anti-IL-2 or anti-CD25 monoclonal antibodies during hapten sensitization increased hapten-specific effector CD8⁺ T cells as well as the magnitude and duration of the CHS response. These results indicate that CD8⁺ T cells are the primary source of IL-2 and that this IL-2 is required for the function of a population of CD4⁺CD25⁺ T cells to restrict the development of the hapten-reactive effector CD8⁺ T cells that mediate CHS responses.

Key Words: delayed-type hypersensitivity • T regulatory cells • skin • T cell priming

This article has been cited by other articles:

				D. D. Kish, A. V. Gorbachev, and R. L. Fairchild Regulatory function of CD4+CD25+ T cells from Class II MHC-deficient mice in contact hypersensitivity responses J. Leukoc. Biol., July 1, 2007; 82(1): 85 - 92. [Abstract] [Full Text] [PDF]