Journal of Leukocyte Biology eBioscience full spectrum cell analysis
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published online as doi:10.1189/jlb.1104631 on June 16, 2005

Published online before print June 16, 2005
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jlb.1104631v1
78/3/656    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Feili-Hariri, M.
Right arrow Articles by Morel, P. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Feili-Hariri, M.
Right arrow Articles by Morel, P. A.
(Journal of Leukocyte Biology. 2005;78:656-664.)
© 2005 by Society for Leukocyte Biology

Polarization of naive T cells into Th1 or Th2 by distinct cytokine-driven murine dendritic cell populations: implications for immunotherapy

Maryam Feili-Hariri*,{dagger},1, Dewayne H. Falkner{dagger} and Penelope A. Morel{dagger},{ddagger},2

* Departments of Surgery
{dagger} Immunology, and
{ddagger} Medicine, University of Pittsburgh School of Medicine, Pennsylvania

1Correspondence: Department of Surgery, Division of Plastic Surgery, University of Pittsburgh, Scaife Hall, Suite 666, 3550 Terrace St., Pittsburgh, PA 15261. E-mail: haririmf{at}upmc.edu

Dendritic cells (DCs) activate T cells and regulate their differentiation into T helper cell type 1 (Th1) and/or Th2 cells. To identify DCs with differing abilities to direct Th1/Th2 cell differentiation, we cultured mouse bone marrow progenitors in granulocyte macrophage-colony stimulating factor (GM), GM + interleukin (IL)-4, or GM + IL-15 and generated three distinct DC populations. The GM + IL-4 DCs expressed high levels of CD80/CD86 and major histocompatibility complex (MHC) class II and produced low levels of IL-12p70. GM and GM + IL-15 DCs expressed low levels of CD80/CD86 and MHC class II. The GM + IL-15 DCs produced high levels of IL-12p70 and interferon (IFN)-{gamma}, whereas GM DCs produced only high levels of IL-12p70. Naive T cells stimulated with GM + IL-4 DCs secreted high levels of IL-4 and IL-5 in addition to IFN-{gamma}. In contrast, the GM + IL-15 DCs induced higher IFN-{gamma} production by T cells with little or no Th2 cytokines. GM DCs did not induce T cell polarization, despite producing large amounts of IL-12p70 following activation. A similar pattern of T cell activation was observed after in vivo administration of DCs. These data suggest that IL-12p70 production alone, although necessary for Th1 differentiation, is not sufficient to induce Th1 responses. These studies have implications for the use of DC-based vaccines in immunotherapy of cancer and other clinical conditions.

Key Words: T cell activation • costimulation • IL-12p70 • IFN-{gamma}




This article has been cited by other articles:


Home page
 J. Immunol.Home page
N. Sachdeva, V. Asthana, T. H. Brewer, D. Garcia, and D. Asthana
Impaired Restoration of Plasmacytoid Dendritic Cells in HIV-1-Infected Patients with Poor CD4 T Cell Reconstitution Is Associated with Decrease in Capacity to Produce IFN-{alpha} but Not Proinflammatory Cytokines
J. Immunol., August 15, 2008; 181(4): 2887 - 2897.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
R. A. Moulton, M. A. Mashruwala, A. K. Smith, D. R. Lindsey, R. A. Wetsel, D. L. Haviland, R. L. Hunter, and C. Jagannath
Complement C5a anaphylatoxin is an innate determinant of dendritic cell-induced Th1 immunity to Mycobacterium bovis BCG infection in mice
J. Leukoc. Biol., October 1, 2007; 82(4): 956 - 967.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
V. Touitou, C. Daussy, B. Bodaghi, S. Camelo, Y. de Kozak, P. Lehoang, M.-C. Naud, A. Varin, B. Thillaye-Goldenberg, H. Merle-Beral, et al.
Impaired Th1/Tc1 Cytokine Production of Tumor-Infiltrating Lymphocytes in a Model of Primary Intraocular B-Cell Lymphoma
Invest. Ophthalmol. Vis. Sci., July 1, 2007; 48(7): 3223 - 3229.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. S. Turner, P. A. Cohen, and O. J. Finn
Lack of Effective MUC1 Tumor Antigen-Specific Immunity in MUC1-Transgenic Mice Results from a Th/T Regulatory Cell Imbalance That Can Be Corrected by Adoptive Transfer of Wild-Type Th Cells
J. Immunol., March 1, 2007; 178(5): 2787 - 2793.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. L. Combe, M. M. Moretto, J. D. Schwartzman, J. P. Gigley, D. J. Bzik, and I. A. Khan
Lack of IL-15 results in the suboptimal priming of CD4+ T cell response against an intracellular parasite
PNAS, April 25, 2006; 103(17): 6635 - 6640.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
D. A. Hokey, A. T. Larregina, G. Erdos, S. C. Watkins, and L. D. Falo Jr.
Tumor Cell Loaded Type-1 Polarized Dendritic Cells Induce Th1-Mediated Tumor Immunity
Cancer Res., November 1, 2005; 65(21): 10059 - 10067.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the Society for Leukocyte Biology.