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Originally published online as doi:10.1189/jlb.0804441 on December 15, 2004

Published online before print December 15, 2004
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(Journal of Leukocyte Biology. 2005;77:337-343.)
© 2005 by Society for Leukocyte Biology

The dendritic cell-derived protein DC-STAMP is highly conserved and localizes to the endoplasmic reticulum

Dagmar Eleveld-Trancikova*, Vassilis Triantis*, Veronique Moulin*, Maaike W. G. Looman*, Mietske Wijers{dagger}, Jack A. M. Fransen{dagger}, Angelique A. C. Lemckert{ddagger}, Menzo J. E. Havenga{ddagger}, Carl G. Figdor*, Richard A. J. Janssen* and Gosse J. Adema*,1

* Departments of Tumor Immunology and
{dagger} Cell Biology, Nijmegen Center for Molecular Life Sciences, University Medical Center Nijmegen, The Netherlands; and
{ddagger} Crucell Leiden, The Netherlands

1 Correspondence: Department of Tumor Immunology, NCMLS/187 TIL University Medical Center, Postbox 9101, 6500HB Nijmegen, The Netherlands. E-mail: g.adema{at}ncmls.kun.nl

Recently, we described the molecular identification of dendritic cell-specific TrAnsMembrane protein (DC-STAMP), a multimembrane-spanning protein preferentially expressed by human DC (hDC). In this report, we describe the identification and expression profile of the murine homologue of DC-STAMP (mDC-STAMP) as well as the characterization of the DC-STAMP protein. The results demonstrate that mDC-STAMP is over 90% homologous to hDC-STAMP and is also preferentially expressed by DC in vitro and ex vivo. mDC-STAMP expression is enhanced by interleukin-4 and down-regulated upon DC maturation. Analysis of differently tagged DC-STAMP proteins further demonstrates that hDC-STAMP and mDC-STAMP are glycosylated and primarily localize to an intracellular compartment. Applying confocal microscopy and electron microscopy, we demonstrate that hDC-STAMP localizes to the endoplasmic reticulum (ER) in human embryonic kidney 293 cells as well as hDC transduced with an adenovirus encoding hDC-STAMP-green fluorescent protein fusion protein. These data imply that DC-STAMP may exert its effect in the ER.

Key Words: mouse • human • APC • CLSM • immunobiology




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