Journal of Leukocyte Biology BioLegend: Treg, Th17, Stem Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published online as doi:10.1189/jlb.0604337 on September 30, 2004

Published online before print September 30, 2004
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jlb.0604337v1
76/6/1220    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McIntire, R. H.
Right arrow Articles by Hunt, J. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McIntire, R. H.
Right arrow Articles by Hunt, J. S.
(Journal of Leukocyte Biology. 2004;76:1220-1228.)
© 2004 by Society for Leukocyte Biology

Recombinant HLA-G5 and -G6 drive U937 myelomonocytic cell production of TGF-ß1

Ramsey H. McIntire*, Pedro J. Morales*, Margaret G. Petroff*, Marco Colonna{dagger} and Joan S. Hunt*,{ddagger},1

* Departments of Anatomy and Cell Biology and
{ddagger} Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas; and
{dagger} Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri

1 Correspondence: Department of Anatomy and Cell Biology, Mail Stop 3038, University of Kansas Medical Center, 3901 Rainbow Blvd., Kansas City, KS 66160. E-mail: jhunt{at}kumc.edu

Throughout human pregnancy, activated maternal macrophages producing anti-inflammatory cytokines comprise a stable cell population in the uterus. This organ is also massively infiltrated with semiallogeneic, placenta-derived, invasive cytotrophoblast cells, which produce membrane and soluble isoforms of human leukocyte antigen (HLA)-G. Here, we investigated the possibility that two soluble isoforms of HLA-G, HLA-G5 and -G6, program macrophage production of cytokines. The model system consisted of human U937 myelomonocytic cells treated with phorbol 12-myristate 13-acetate (PMA) and interferon-{gamma} (IFN-{gamma}), which induced differentiation and activation but did not affect their viability or decrease their expression of the two inhibitory immunoglobulin-like transcript (ILT) receptors for HLA-G, ILT2 and ILT4. Exposure of the PMA/IFN-{gamma}-treated U937 cells to increasing concentrations of recombinant HLA-G5 or -G6 (rG5 and rG6) stimulated effects common to the two isoforms. High doses of both significantly decreased interleukin (IL)-10 and dramatically increased transforming growth factor-ß1. Differential effectiveness between the isoforms was demonstrated in dose-response studies, as was differential binding to ILT2 and ILT4 in receptor-blocking studies. No effects on production of IL-4, IL-1 receptor antagonist, IL-15, tumor necrosis factor {alpha}, IL-1ß, or IL-6 were observed. Collectively, the results are consistent with the postulate that environmental programming of decidual macrophages may be dictated in part by their proximity to soluble HLA-G-producing fetal cytotrophoblast cells.

Key Words: human • macrophage cytokines • pregnancy immunology




This article has been cited by other articles:


Home page
 Reproductive SciencesHome page
R. H. McIntire, K. G. Ganacias, and J. S. Hunt
Programming of Human Monocytes by the Uteroplacental Environment
Reproductive Sciences, May 1, 2008; 15(5): 437 - 447.
[Abstract] [PDF]

Home page
 J. Immunol.Home page
G. I. Bondarenko, D. W. Burleigh, M. Durning, E. E. Breburda, R. L. Grendell, and T. G. Golos
Passive Immunization against the MHC Class I Molecule Mamu-AG Disrupts Rhesus Placental Development and Endometrial Responses
J. Immunol., December 15, 2007; 179(12): 8042 - 8050.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. Naji, S. Le Rond, A. Durrbach, I. Krawice-Radanne, C. Creput, M. Daouya, J. Caumartin, J. LeMaoult, E. D. Carosella, and N. Rouas-Freiss
CD3+CD4low and CD3+CD8low are induced by HLA-G: novel human peripheral blood suppressor T-cell subsets involved in transplant acceptance
Blood, December 1, 2007; 110(12): 3936 - 3948.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
T. V. F. Hviid
HLA-G in human reproduction: aspects of genetics, function and pregnancy complications
Hum. Reprod. Update, May 1, 2006; 12(3): 209 - 232.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Lafon, C. Prehaud, F. Megret, M. Lafage, G. Mouillot, M. Roa, P. Moreau, N. Rouas-Freiss, and E. D. Carosella
Modulation of HLA-G Expression in Human Neural Cells after Neurotropic Viral Infections
J. Virol., December 15, 2005; 79(24): 15226 - 15237.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
H. Wiendl, U. Feger, M. Mittelbronn, C. Jack, B. Schreiner, C. Stadelmann, J. Antel, W. Brueck, R. Meyermann, A. Bar-Or, et al.
Expression of the immune-tolerogenic major histocompatibility molecule HLA-G in multiple sclerosis: implications for CNS immunity
Brain, November 1, 2005; 128(11): 2689 - 2704.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. Le Discorde, C. Le Danff, P. Moreau, N. Rouas-Freiss, and E. D. Carosella
HLA-G*0105N Null Allele Encodes Functional HLA-G Isoforms
Biol Reprod, August 1, 2005; 73(2): 280 - 288.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
J. S. Hunt, M. G. Petroff, R. H. McIntire, and C. Ober
HLA-G and immune tolerance in pregnancy
FASEB J, May 1, 2005; 19(7): 681 - 693.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Society for Leukocyte Biology.