| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Published online before print August 17, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Physiology Program, Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts
1 Correspondence: Harvard School of Public Health, 665 Huntington Avenue, SPH-2, Room 223, Boston, MA 02115. E-mail: sjozefow{at}hsph.harvard.edu
Although class A type I/II scavenger receptor (SR-A) is involved in numerous macrophage functions, its signaling ability remains uncertain. We used monoclonal antibodies (mAb) to specifically stimulate receptors on mouse alveolar (AMs) and peritoneal macrophages (PMs). Immobilized anti-SR-A (2F8) and anti-Fc
R II/III (2.4G2) mAb stimulated hydrogen peroxide (H2O2) production in normal C3H/HeJ AMs (by 55% and 98%, respectively) and resident PMs (66% and 128%). The 2F8 mAb-stimulated H2O2 production resulted from specific stimulation of SR-A, since this response was absent in AMs from SR-A-deficient or C57BL/6 mice—the latter strain expressing an allelic form of SR-A, unrecognizable by 2F8 mAb. H2O2 production stimulated by anti-SR-A but not by anti-FcRII/III mAb was preserved in FcRI/III-deficient mice, ruling out involvement of FcRs in the 2F8 mAb effect. In comparison with the FcR-stimulated respiratory burst, the response to anti-SR-A mAb was delayed and, unlike the former, inhibited by pertussis toxin. Ligation of SR-A also inhibited lipopolysaccharide plus interferon--stimulated interleukin-12 (IL-12) release, by 25% in AMs and by 68% in thioglycollate-elicited PMs, consistent with different levels of SR-A expression. Neither nitrite nor IL-6 accumulation was affected by anti-SR-A mAb. SR-A-stimulated H2O2 does not seem to mediate the inhibition of IL-12 release, since the inhibition was neither reversed by scavenging of H2O2 nor mimicked by exogenous H2O2. Our results indicate that SR-A not only mediates endocytosis but can also generate signals such as H2O2, which may affect microbicidal or proinflammatory functions.
Key Words: Fc receptors • knockout • alveolar • peritoneal • hydrogen peroxide
This article has been cited by other articles:
|
|
 |
|
  J. C. Todt, B. Hu, and J. L. Curtis The scavenger receptor SR-A I/II (CD204) signals via the receptor tyrosine kinase Mertk during apoptotic cell uptake by murine macrophages J. Leukoc. Biol., August 1, 2008; 84(2): 510 - 518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Nakao, T. Kurokawa, T. Nonami, G. Tumurkhuu, N. Koide, and T. Yokochi Hydrogen peroxide induces the production of tumor necrosis factor-{alpha} in RAW 264.7 macrophage cells via activation of p38 and stress-activated protein kinase Innate Immunity, June 1, 2008; 14(3): 190 - 196. [Abstract] [PDF]
|
|
|
|
 |
|
 P. Haberzettl, R. P. F. Schins, D. Hohr, V. Wilhelmi, P. J. A. Borm, and C. Albrecht Impact of the Fc{gamma}II-receptor on quartz uptake and inflammatory response by alveolar macrophages Am J Physiol Lung Cell Mol Physiol, June 1, 2008; 294(6): L1137 - L1148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Okamura, J. M. Lopez-Guisa, K. Koelsch, S. Collins, and A. A. Eddy Atherogenic scavenger receptor modulation in the tubulointerstitium in response to chronic renal injury Am J Physiol Renal Physiol, August 1, 2007; 293(2): F575 - F585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-Y. Wang, J. Facciponte, X. Chen, J. R. Subjeck, and E. A. Repasky Scavenger Receptor-A Negatively Regulates Antitumor Immunity Cancer Res., May 15, 2007; 67(10): 4996 - 5002. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. K. Usui, K. Shikata, M. Sasaki, S. Okada, M. Matsuda, Y. Shikata, D. Ogawa, Y. Kido, R. Nagase, K. Yozai, et al. Macrophage Scavenger Receptor-A-Deficient Mice Are Resistant Against Diabetic Nephropathy Through Amelioration of Microinflammation Diabetes, February 1, 2007; 56(2): 363 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jozefowski, T. H. Sulahian, M. Arredouani, and L. Kobzik Role of scavenger receptor MARCO in macrophage responses to CpG oligodeoxynucleotides J. Leukoc. Biol., October 1, 2006; 80(4): 870 - 879. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Arredouani, Z. Yang, A. Imrich, Y. Ning, G. Qin, and L. Kobzik The Macrophage Scavenger Receptor SR-AI/II and Lung Defense against Pneumococci and Particles Am. J. Respir. Cell Mol. Biol., October 1, 2006; 35(4): 474 - 478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Fronhofer, M. R. Lennartz, and D. J. Loegering Role of PKC isoforms in the Fc{gamma}R-mediated inhibition of LPS-stimulated IL-12 secretion by macrophages J. Leukoc. Biol., February 1, 2006; 79(2): 408 - 415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jozefowski, M. Arredouani, T. Sulahian, and L. Kobzik Disparate Regulation and Function of the Class A Scavenger Receptors SR-AI/II and MARCO J. Immunol., December 15, 2005; 175(12): 8032 - 8041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Beamer and A. Holian Scavenger receptor class A type I/II (CD204) null mice fail to develop fibrosis following silica exposure Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L186 - L195. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
