HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Published online before print April 23, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: jlb.0104031v1 76/1/86    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Reid, D. M. Articles by Wong, S. Y. C. Search for Related Content PubMed PubMed Citation Articles by Reid, D. M. Articles by Wong, S. Y. C.

(Journal of Leukocyte Biology. 2004;76:86-94.)
© 2004 by Society for Leukocyte Biology

Expression of the ß-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions

Delyth M. Reid^*,1, Maria Montoya^*, Philip R. Taylor, Persephone Borrow^*, Siamon Gordon, Gordon D. Brown and Simon Y. C. Wong^*

^* The Edward Jenner Institute for Vaccine Research, Compton, Berkshire, United Kingdom; and
Sir William Dunn School of Pathology, Oxford University, United Kingdom

¹Correspondence: The Edward Jenner Institute for Vaccine Research, Compton, Berkshire, RG20 7NN, UK. E-mail: delyth.wong{at}jenner.ac.uk

Dectin-1 is a pathogen-recognition receptor on macrophages (Ms), neutrophils, and dendritic cells (DCs). On Ms and bone marrow-derived DCs, it has been shown to mediate the nonopsonic recognition of and response to soluble and particulate yeast ß-glucans. We have optimized the immunohistochemical detection of Dectin-1 and demonstrated its expression on neutrophils, subpopulations of Ms in splenic red and white pulp, alveolar Ms, Kupffer cells, and Ms and DCs in the lamina propria of gut villi. This is consistent with its role in pathogen surveillance. A significant proportion of CD11c⁺ splenic DCs expressed Dectin-1, but expression was not restricted to any one subset. Dectin-1 expression was low on resident Ms and DCs of skin and was not detected on resident Ms or DCs in kidney, heart, brain, or eye. The proposed, additional role of Dectin-1 as a coreceptor for T cell activation is supported by its expression on DCs in the T cell areas of the spleen and lymph nodes. Strong expression of Dectin-1 on subpopulations of Ms and DCs in the medullary and corticomedullary regions of the thymus suggests a role distinct from pathogen recognition. Tissue localization thus revealed potential roles of Dectin-1 in leukocyte interactions during innate immune responses and T cell development.

Key Words: innate immunity • macrophages • dendritic cells • spleen • thymus development

This article has been cited by other articles:

				A. B. Tarabishy, B. Aldabagh, Y. Sun, Y. Imamura, P. K. Mukherjee, J. H. Lass, M. A. Ghannoum, and E. Pearlman MyD88 Regulation of Fusarium Keratitis Is Dependent on TLR4 and IL-1R1 but Not TLR2 J. Immunol., July 1, 2008; 181(1): 593 - 600. [Abstract] [Full Text] [PDF]

				C. A. Wells, J. A. Salvage-Jones, X. Li, K. Hitchens, S. Butcher, R. Z. Murray, A. G. Beckhouse, Y.-L.-S. Lo, S. Manzanero, C. Cobbold, et al. The Macrophage-Inducible C-Type Lectin, Mincle, Is an Essential Component of the Innate Immune Response to Candida albicans J. Immunol., June 1, 2008; 180(11): 7404 - 7413. [Abstract] [Full Text] [PDF]

				A. Koneti, M. J. Linke, E. Brummer, and D. A. Stevens Evasion of Innate Immune Responses: Evidence for Mannose Binding Lectin Inhibition of Tumor Necrosis Factor Alpha Production by Macrophages in Response to Blastomyces dermatitidis Infect. Immun., March 1, 2008; 76(3): 994 - 1002. [Abstract] [Full Text] [PDF]

				A. G. Rothfuchs, A. Bafica, C. G. Feng, J. G. Egen, D. L. Williams, G. D. Brown, and A. Sher Dectin-1 Interaction with Mycobacterium tuberculosis Leads to Enhanced IL-12p40 Production by Splenic Dendritic Cells J. Immunol., September 15, 2007; 179(6): 3463 - 3471. [Abstract] [Full Text] [PDF]

				D. Vermijlen, P. Ellis, C. Langford, A. Klein, R. Engel, K. Willimann, H. Jomaa, A. C. Hayday, and M. Eberl Distinct Cytokine-Driven Responses of Activated Blood {gamma}{delta} T Cells: Insights into Unconventional T Cell Pleiotropy J. Immunol., April 1, 2007; 178(7): 4304 - 4314. [Abstract] [Full Text] [PDF]

				F. Gays, J. G. Aust, D. M. Reid, J. Falconer, N. Toyama-Sorimachi, P. R. Taylor, and C. G. Brooks Ly49B Is Expressed on Multiple Subpopulations of Myeloid Cells J. Immunol., November 1, 2006; 177(9): 5840 - 5851. [Abstract] [Full Text] [PDF]

				R. W. Carter, C. Thompson, D. M. Reid, S. Y. C. Wong, and D. F. Tough Preferential Induction of CD4+ T Cell Responses through In Vivo Targeting of Antigen to Dendritic Cell-Associated C-Type Lectin-1 J. Immunol., August 15, 2006; 177(4): 2276 - 2284. [Abstract] [Full Text] [PDF]

				A. S. Palma, T. Feizi, Y. Zhang, M. S. Stoll, A. M. Lawson, E. Diaz-Rodriguez, M. A. Campanero-Rhodes, J. Costa, S. Gordon, G. D. Brown, et al. Ligands for the beta-Glucan Receptor, Dectin-1, Assigned Using "Designer" Microarrays of Oligosaccharide Probes (Neoglycolipids) Generated from Glucan Polysaccharides J. Biol. Chem., March 3, 2006; 281(9): 5771 - 5779. [Abstract] [Full Text] [PDF]

				S. Suram, G. D. Brown, M. Ghosh, S. Gordon, R. Loper, P. R. Taylor, S. Akira, S. Uematsu, D. L. Williams, and C. C. Leslie Regulation of Cytosolic Phospholipase A2 Activation and Cyclooxygenase 2 Expression in Macrophages by the beta-Glucan Receptor J. Biol. Chem., March 3, 2006; 281(9): 5506 - 5514. [Abstract] [Full Text] [PDF]

				L. C. Parker, M. K. B. Whyte, S. K. Dower, and I. Sabroe The expression and roles of Toll-like receptors in the biology of the human neutrophil J. Leukoc. Biol., June 1, 2005; 77(6): 886 - 892. [Abstract] [Full Text] [PDF]

				S. M. Moghimi, A. C. Hunter, and J. C. Murray Nanomedicine: current status and future prospects FASEB J, March 1, 2005; 19(3): 311 - 330. [Abstract] [Full Text] [PDF]