Published online before print February 24, 2004
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* INSERM U396, Institut des Cordeliers, Paris, France;
Department of Immunology, Institut Pasteur, Paris, France; and
Department of Immunology, Royal Postgraduate Medical School, Hammersmith Hospital and University College London, United Kingdom
1 Correspondence: INSERM U396, Institut des Cordeliers, 75006, Paris, France. E-mail: nuala.mooney{at}bhdc.jussieu.fr
Antigen presentation to T lymphocytes has been characterized extensively in terms of T lymphocyte activation and eventual cell death. In contrast, little is known about the consequences of antigen presentation for the antigen-presenting cell (APC). We have determined the outcome of major histocompatibility complex class II-restricted peptide presentation to a specific T cell. We demonstrate that specific T lymphocyte interaction with peptide-presenting APCs led to apoptosis in the APC population. In contrast, T lymphocyte interaction with nonpeptide-loaded APCs or APCs loaded with monosubstituted peptide failed to induce T lymphocyte secretion of interleukin-2 and APC apoptosis. Phosphatidylserine externalization and mitochondrial depolarization were used to evaluate APC apoptosis. Fas/Fas ligand interactions were not required, but cytoskeletal integrity and caspase activation were essential for APC apoptosis. Antigen presentation leading to T lymphocyte activation is therefore coordinated with apoptosis in the APC population and could provide a mechanism of immune response regulation by eliminating APCs, which have fulfilled their role as specific ligands for T lymphocyte activation. This pathway may have particular importance for APCs, which are not sensitive to death receptor-induced apoptosis.
Key Words: MHC class II • T–B interaction • programmed cell death
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