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Published online before print March 12, 2004
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* Deutsches Rheuma-Forschungszentrum, Berlin, Germany;
Miltenyi Biotec GmbH, Bergisch Gladbach, Germany;
Charite’ Humboldt University, Berlin, Germany; and
Orthopädische Klinik Braunschweig, Germany
1 Correspondence: Deutsches Rheuma-Forschungszentrum, Berlin, Schumannstrasse 20/21, D-10117 Berlin, Germany. E-mail: manz{at}drfz.de
Despite the important role immunoglobulin G (IgG)-secreting plasma cells play in memory immune responses, the differentiation and homeostasis of these cells are not completely understood. Here, we studied the differentiation of human IgG-secreting cells ex vivo and in vitro, identifying these cells by the cellular affinity matrix technology. Several subpopulations of IgG-secreting cells were identified among the cells isolated from tonsils and bone marrow, particularly differing in the expression levels of CD9, CD19, and CD38. CD38 low IgG-secreting cells were present exclusively in the tonsils. A major fraction of these cells appeared to be early plasma cell precursors, as upon activation of B cells in vitro, IgG secretion preceded up-regulation of CD38, and on tonsillar sections, IgG-containing, CD38 low cells with a plasmacytoid phenotype were found in follicles, where plasma cell differentiation starts. A unitary phenotype of migratory peripheral blood IgG-secreting cells suggests that all bone marrow plasma cell populations share a common precursor cell. These data are compatible with a multistep model for plasma cell differentiation and imply that a common CD38 low IgG-secreting precursor gives rise to a diverse plasma cell compartment.
Key Words: antibodies • B lymphocytes • cellular differentiation • memory
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