HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Published online before print January 2, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: jlb.0902471v1 75/4/657    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Workalemahu, G. Articles by Kroegel, C. Search for Related Content PubMed PubMed Citation Articles by Workalemahu, G. Articles by Kroegel, C.

(Journal of Leukocyte Biology. 2004;75:657-663.)
© 2004 by Society for Leukocyte Biology

Expression and synthesis of fibroblast growth factor-9 in human T-lymphocytes. Response to isopentenyl pyrophosphate and TGF-ß1/IL-15

University Medical Clinic I, Department of Pneumology and Allergy/Immunology, Friedrich-Schiller-University Jena, Germany

¹Correspondence: Pneumology, Medical Clinic I, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, Germany. E-mail: grefachew.workalemahu{at}med.uni-jena.de

T-lymphocytes are believed to play a role in maintaining the normal configuration of epithelial tissue. As little is known about the factors mediating this function, we addressed the question of whether T-lymphocytes produce fibroblast growth factor (FGF)-9 as well as two other growth factors associated with epithelial tissue reconstitution. Blood T cells isolated from healthy donors were grown in the presence of isopentenyl pyrophosphate (IPP) or transforming growth factor-ß1 (TGF-ß1)/interleukin-15 (IL-15) for 24 h and were assessed for the expression and synthesis of FGF-9, keratinocyte growth factor (KGF), and epidermal growth factor (EGF). Resting human T cells constitutively expressed KGF and FGF-9 mRNA but no EGF mRNA. In the presence of IPP, FGF-9 mRNA expression significantly increased in a dose-dependent manner, expression of KGF remained unaltered, and EGF mRNA could not be detected. In contrast to IPP, stimulation of the cells with TGF-ß1/IL-15 did not alter FGF-9 expression. Moreover, stimulation with anti-CD3 does not induce FGF-9 expression but triggers a high signal of interferon- mRNA. Western blot analysis of T cell lysates, prepared 4 days following stimulation with IPP, showed an increase of FGF-9 protein as compared with control cells. In conclusion, the results demonstrate for the first time that human blood and bronchoalveolar lavage T-lymphocytes are capable of expressing FGF-9. The data also provide novel evidence that immunoregulatory cells can synthesize FGF-9.

Key Words: growth factors • bacterial peptide • IPP

This article has been cited by other articles:

				Y. Omoto Estrogen Receptor-{alpha} Signaling in Growth of the Ventral Prostate: Comparison of Neonatal Growth and Postcastration Regrowth Endocrinology, September 1, 2008; 149(9): 4421 - 4427. [Abstract] [Full Text] [PDF]

				Y. Kagimoto, H. Yamada, T. Ishikawa, N. Maeda, F. Goshima, Y. Nishiyama, M. Furue, and Y. Yoshikai A regulatory role of interleukin 15 in wound healing and mucosal infection in mice J. Leukoc. Biol., January 1, 2008; 83(1): 165 - 172. [Abstract] [Full Text] [PDF]

				K.-J. Puan, C. Jin, H. Wang, G. Sarikonda, A. M. Raker, H. K. Lee, M. I. Samuelson, E. Marker-Hermann, L. Pasa-Tolic, E. Nieves, et al. Preferential recognition of a microbial metabolite by human V{gamma}2V{delta}2 T cells Int. Immunol., May 1, 2007; 19(5): 657 - 673. [Abstract] [Full Text] [PDF]

				D. Kabelitz, D. Wesch, E. Pitters, and M. Zoller Characterization of Tumor Reactivity of Human V{gamma}9V{delta}2 {gamma}{delta} T Cells In Vitro and in SCID Mice In Vivo J. Immunol., December 1, 2004; 173(11): 6767 - 6776. [Abstract] [Full Text] [PDF]