Pepro Tech
Originally published online as doi:10.1189/jlb.0603250 on December 12, 2003

Published online before print December 12, 2003
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
jlb.0603250v1
75/3/434    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kobayashi, H.
Right arrow Articles by Ishii, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kobayashi, H.
Right arrow Articles by Ishii, H.
(Journal of Leukocyte Biology. 2004;75:434-442.)
© 2004 by Society for Leukocyte Biology

In situ demonstration of dendritic cell migration from rat intestine to mesenteric lymph nodes: relationships to maturation and role of chemokines

Hisashi Kobayashi*,1, Soichiro Miura{dagger}, Hiroshi Nagata*, Yoshikazu Tsuzuki{dagger}, Ryota Hokari{dagger}, Takashi Ogino*, Chikako Watanabe*, Toshifumi Azuma* and Hiromasa Ishii*

* Department of Internal Medicine, Keio University, School of Medicine, Tokyo, Japan; and
{dagger} Second Department of Internal Medicine, National Defense Medical College, Saitama, Japan

1 Correspondence: Second Department of Internal Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan. E-mail: miura{at}me.ndmc.ac.jp

Dendritic cells (DCs) are continuously transported from the intestine to mesenteric lymph nodes (MLNs). The objective of this study was to determine the migration kinetics of DCs via intestinal lymph and to investigate regulatory factors affecting their migration in vivo. DCs were obtained from spleen or thoracic duct lymph of mesenteric lymphadenectomized rats. The DCs were fluorescently labeled and injected into the subserosa of the small intestine near the cecum, and their migration patterns into MLNs were determined. Isolated DCs from intestinal lymph express intercellular adhesion molecule-1 (ICAM-1), CD11b/c, CD80/86, and major histocompatibility complex class II but maintain their ability to phagocytize latex particles, suggesting the presence of immature DCs. The isolated DCs accumulated in MLNs in a time-dependent manner with maximal accumulation at 48 h. Cytokine-induced maturation of lymph DCs did not cause a change in cell number but accelerated their transport into MLNs with a maximum at 24 h. Splenic DCs showed an intermediate level of maturation and a migration pattern similar to mature DCs. Inhibition of ICAM-1 or CD11b/c did not affect DC migration. Migration of mature DCs to MLNs was specifically blocked by desensitization of CCR7 with CCL21. In contrast, freshly isolated lymph DCs were not chemotactic for CCL21, but their migration to MLNs was mainly inhibited by desensitization of CCR6 with CCL20. The migratory ability of DCs correlates well with their degree of maturation, and different chemokine/chemokine receptor use may be the main regulator of DC migration kinetics through intestinal lymph.

Key Words: intestinal lymph • spleen • adhesion molecule • CCL20 • CCL21 • CCR6 • CCR7 • CXCL12




This article has been cited by other articles:


Home page
 J. Immunol.Home page
A. Pegu, S. Qin, B. A. Fallert Junecko, R. E. Nisato, M. S. Pepper, and T. A. Reinhart
Human Lymphatic Endothelial Cells Express Multiple Functional TLRs
J. Immunol., March 1, 2008; 180(5): 3399 - 3405.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
C. Porporatto, I. D. Bianco, and S. G. Correa
Local and systemic activity of the polysaccharide chitosan at lymphoid tissues after oral administration
J. Leukoc. Biol., July 1, 2005; 78(1): 62 - 69.
[Abstract] [Full Text] [PDF]