| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Published online before print November 3, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
+ and CD8- dendritic cells and their in vivo trafficking
,,1
* Thomas E. Starzl Transplantation Institute and Departments of
Surgery and
Immunology, University of Pittsburgh Medical Center, Pennsylvania
1 Correspondence: W1544 Biomedical Science Tower, University of Pittsburgh Medical Center, 200 Lothrop St., Pittsburgh, PA 15213. E-mail: thomsonaw{at}msx.upmc.edu
Murine CD11c+CD8
- and CD11c+CD8+ dendritic cells (DCs) differentially regulate T cell responses. Although specific chemokines that recruit immature (i) or mature (m) CD8- DCs have been identified, little is known about the influence of chemokines on CD8+ DCs. iDCs and mDCs isolated from spleens of fms-like tyrosine kinase 3 ligand-treated B10 mice were compared directly for migratory responses to a panel of CC chemokines or following local or systemic administration. In vitro assays were performed using Transwell® chambers. iDCs did not respond to any CC chemokines tested. Both subsets of mDCs migrated to CCL19 and CCL21, with consistently lower percentages of CD8+ DCs migrating. Chemokine receptor mRNA and protein expression were analyzed, but no correlation between expression and function was demonstrated. In vivo trafficking of fluorochrome-labeled DCs (B10; H2b) was assessed by immunohistochemistry and by rare-event flow cytometric analysis of allogeneic recipient (BALB/c; H2d) draining lymph node (DLN) and spleen cells. Twenty-four hours after intravenous injection, chloromethylfluorescein diacetate-positive CD8+ and CD8- mDCs were detected by immunohistochemistry in spleens in similar numbers (that decreased over time). Following subcutaneous injection, both DC subsets were detected in DLN at 24 h, but only CD8- DCs were evident by flow analysis at 48 h. Although CD8+ DCs migrate from peripheral tissues to T cell areas of (allogeneic) secondary lymphoid organs, they appear to mobilize as mDCs and less efficiently than CD8- mDCs.
Key Words: leukocyte chemotaxis • antigen-presenting cell • lymphoid tissue
This article has been cited by other articles:
|
|
 |
|
  Q. Sun, C. T. Kong, F. P. Huang, and L. C. Chan Aberrant dendritic cell differentiation initiated by the Mll-Een fusion gene does not require leukemic transformation J. Leukoc. Biol., January 1, 2008; 83(1): 173 - 180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Czeloth, A. Schippers, N. Wagner, W. Muller, B. Kuster, G. Bernhardt, and R. Forster Sphingosine-1 Phosphate Signaling Regulates Positioning of Dendritic Cells within the Spleen J. Immunol., November 1, 2007; 179(9): 5855 - 5863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Bertram, S. Baltic, N. L. Misso, K. D. Bhoola, P. S. Foster, P. J. Thompson, and M. Fogel-Petrovic Expression of kinin B1 and B2 receptors in immature, monocyte-derived dendritic cells and bradykinin-mediated increase in intracellular Ca2+ and cell migration J. Leukoc. Biol., June 1, 2007; 81(6): 1445 - 1454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Y. Lan, Z. Wang, G. Raimondi, W. Wu, B. L. Colvin, A. De Creus, and A. W. Thomson "Alternatively Activated" Dendritic Cells Preferentially Secrete IL-10, Expand Foxp3+CD4+ T Cells, and Induce Long-Term Organ Allograft Survival in Combination with CTLA4-Ig J. Immunol., November 1, 2006; 177(9): 5868 - 5877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. H. Schimmelpfennig, S. Schulz, C. Arber, J. Baker, I. Tarner, J. McBride, C. H. Contag, and R. S. Negrin Ex Vivo Expanded Dendritic Cells Home to T-Cell Zones of Lymphoid Organs and Survive in Vivo after Allogeneic Bone Marrow Transplantation Am. J. Pathol., November 1, 2005; 167(5): 1321 - 1331. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
