Journal of Leukocyte Biology eBioscience full spectrum cell analysis
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Originally published online as doi:10.1189/jlb.0703339 on November 21, 2003

Published online before print November 21, 2003
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(Journal of Leukocyte Biology. 2004;75:240-243.)
© 2004 by Society for Leukocyte Biology

Rapid and extensive membrane reorganization by dendritic cells following exposure to bacteria revealed by high-resolution imaging

Russell D. Salter*,1, Renee J. Tuma-Warrino*, Paul Q. Hu* and Simon C. Watkins*,{dagger}

Departments of
* Immunology and
{dagger} Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pennsylvania

1 Correspondence: E1052 BST, 200 Lothrop Street, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213. E-mail: rds{at}pitt.edu

Using live cell imaging, we demonstrate that immature dendritic cells (DC) derived from human peripheral blood monocytes undergo pronounced morphologic changes in vitro within minutes of exposure to unopsonized Escherichia coli, developing extensive membrane veils that efficiently capture additional bacteria. Internalization does not occur in the veils, but instead, bacteria are transported to the central region of the cell, where they sink directly into the plasma membrane. In contrast, exposure to polystyrene beads does not induce notable changes in cell morphology, and DC do not efficiently capture beads when introduced alone or mixed with bacteria. Long dendritic processes were also visualized in some cells that allowed capture of clumps of bacteria at a distance of more than 100 µm. These results demonstrate that immature DC can distinguish between inert particles and bacteria and alter their shape and phagocytic capacity in response to the latter.

Key Words: E. coli • green fluorescent protein • phagocytosis




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