Published online before print October 2, 2003

This Article Full Text Free Full Text (PDF) Free All Versions of this Article: jlb.0703326v1 75/1/68    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Roark, C. L. Articles by O’Brien, R. L. Search for Related Content PubMed PubMed Citation Articles by Roark, C. L. Articles by O’Brien, R. L.

(Journal of Leukocyte Biology. 2004;75:68-75.)
© 2004 by Society for Leukocyte Biology

Subset-specific, uniform activation among V6/V1⁺ T cells elicited by inflammation

Christina L. Roark^*,1, M. Kemal Aydintug^*,1, Julie Lewis, Xiang Yin^*, Michael Lahn^*, Youn-Soo Hahn^*, Willi K. Born^*, Robert E. Tigelaar and Rebecca L. O’Brien^*,2

^* Integrated Department of Immunology, National Jewish Medical and Research Center, Denver, Colorado; and
Department of Dermatology, Yale University School of Medicine, New Haven, Connecticut

²Correspondence: Integrated Department of Immunology, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206. E-mail: obrienr{at}njc.org

The V6/V1⁺ cells, the second murine T cell subset to arise in the thymus, express a nearly invariant T cell receptor (TCR), colonize select tissues, and expand preferentially in other tissues during inflammation. These cells are thought to help in regulating the inflammatory response. Until now, V6/V1⁺ cells have only been detectable indirectly, by expression of V6-encoding mRNA. Here, we report that 17D1, a monoclonal antibody, which detects the related epidermis-associated V5/V1⁺ TCR, will also bind the V6/V1⁺ cells if their TCR is first complexed to an anti-C antibody. Features of this special condition for recognition suggest the possibility that an alternate structure exists for the V6/V1 TCR, which is stabilized upon binding to the anti-C antibody. Using the 17D1 antibody as means to track this T cell subset by flow cytometry, we discovered that the response of V6/V1⁺ cells during inflammation often far exceeds that of other subsets and that the responding V6/V1⁺ cells display a strikingly uniform activation/memory phenotype compared with other T cell subsets.

Key Words: T cells • T cell receptors

This article has been cited by other articles:

				R. L. O'Brien, M. A. Taylor, J. Hartley, T. Nuhsbaum, S. Dugan, K. Lahmers, M. K. Aydintug, J. M. Wands, C. L. Roark, and W. K. Born Protective Role of {gamma}{delta} T Cells in Spontaneous Ocular Inflammation Invest. Ophthalmol. Vis. Sci., July 1, 2009; 50(7): 3266 - 3274. [Abstract] [Full Text] [PDF]

				P. L. Simonian, C. L. Roark, F. Wehrmann, A. M. Lanham, W. K. Born, R. L. O'Brien, and A. P. Fontenot IL-17A-Expressing T Cells Are Essential for Bacterial Clearance in a Murine Model of Hypersensitivity Pneumonitis J. Immunol., May 15, 2009; 182(10): 6540 - 6549. [Abstract] [Full Text] [PDF]

				C.-H. Kim, D. A. Witherden, and W. L. Havran Characterization and TCR variable region gene use of mouse resident nasal {gamma}{delta} T lymphocytes J. Leukoc. Biol., November 1, 2008; 84(5): 1259 - 1263. [Abstract] [Full Text] [PDF]

				S. Hamada, M. Umemura, T. Shiono, K. Tanaka, A. Yahagi, M. D. Begum, K. Oshiro, Y. Okamoto, H. Watanabe, K. Kawakami, et al. IL-17A Produced by {gamma}{delta} T Cells Plays a Critical Role in Innate Immunity against Listeria monocytogenes Infection in the Liver J. Immunol., September 1, 2008; 181(5): 3456 - 3463. [Abstract] [Full Text] [PDF]

				S. Miyazaki, F. Ishikawa, K. Shimizu, T. Ubagai, P. H. Edelstein, and K. Yamaguchi Gr-1high Polymorphonuclear Leukocytes and NK Cells Act via IL-15 to Clear Intracellular Haemophilus influenzae in Experimental Murine Peritonitis and Pneumonia J. Immunol., October 15, 2007; 179(8): 5407 - 5414. [Abstract] [Full Text] [PDF]

				P. L. Simonian, C. L. Roark, F. Diaz del Valle, B. E. Palmer, I. S. Douglas, K. Ikuta, W. K. Born, R. L. O'Brien, and A. P. Fontenot Regulatory Role of {gamma}{delta} T Cells in the Recruitment of CD4+ and CD8+ T Cells to Lung and Subsequent Pulmonary Fibrosis J. Immunol., October 1, 2006; 177(7): 4436 - 4443. [Abstract] [Full Text] [PDF]

				J. M. Wands, C. L. Roark, M. K. Aydintug, N. Jin, Y.-S. Hahn, L. Cook, X. Yin, J. Dal Porto, M. Lahn, D. M. Hyde, et al. Distribution and leukocyte contacts of {gamma}{delta} T cells in the lung J. Leukoc. Biol., November 1, 2005; 78(5): 1086 - 1096. [Abstract] [Full Text] [PDF]

				N. Jin, C. Taube, L. Sharp, Y.-S. Hahn, X. Yin, J. M. Wands, C. L. Roark, R. L. O'Brien, E. W. Gelfand, and W. K. Born Mismatched Antigen Prepares {gamma}{delta} T Cells for Suppression of Airway Hyperresponsiveness J. Immunol., March 1, 2005; 174(5): 2671 - 2679. [Abstract] [Full Text] [PDF]