HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Published online before print October 2, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: jlb.0403146v1 75/1/135    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cappello, P. Articles by Giovarelli, M. Search for Related Content PubMed PubMed Citation Articles by Cappello, P. Articles by Giovarelli, M.

(Journal of Leukocyte Biology. 2004;75:135-142.)
© 2004 by Society for Leukocyte Biology

CCL16/LEC powerfully triggers effector and antigen-presenting functions of macrophages and enhances T cell cytotoxicity

Department of Clinical and Biological Sciences, University of Turin, Orbassano, Italy; and Center for Experimental Research and Medical Studies (CERMS), S. Giovanni Battista Hospital, Turin, Italy

¹Correspondence: Dipartimento di Scienze Cliniche e Biologiche, Università di Torino, Ospedale San Luigi Gonzaga, 10043 Orbassano, Italy. E-mail: mirella.giovarelli{at}unito.it

The human CC chemokine CCL16, a liver-expressed chemokine, enhances the killing activity of mouse peritoneal macrophages by triggering their expression of tumor necrosis factor (TNF-) and Fas ligand. Macrophages also respond to CCL16 by enhancing their production of monocyte chemoattractant protein-1, regulated on activation, normal T cells expressed and secreted chemokines, and interleukin (IL)-1ß, TNF-, and IL-12. The effect of CCL16 is almost as strong as that of lipopolysaccharide and interferon-, two of the best macrophage activators. Moreover, CCL16-activated macrophages overexpress membrane CD80, CD86, and CD40 costimulatory molecules and extensively phagocytose tumor cell debris. On exposure to such debris, they activate a strong, tumor-specific, cytolytic response in virgin T cells. Furthermore, cytolytic T cells generated in the presence of CCL16 display a higher cytotoxicity and activate caspase-8 in tumor target cells. This ability to activate caspase-8 depends on their overexpression of TNF- and Fas ligand induced by CCL16. These data reveal a new function for CCL16 in the immune-response scenario. CCL16 significantly enhances the effector and the antigen-presenting function of macrophages and augments T cell lytic activity.

Key Words: tumor antigen uptake • T cell priming • costimulatory molecules

This article has been cited by other articles:

				M J Bugeja, D R Booth, B H Bennetts, R N. Heard, and G J Stewart An investigation of polymorphisms in the 4q13.3-21.1 CXC chemokine gene cluster for association with multiple sclerosis in Australians Multiple Sclerosis, November 1, 2006; 12(6): 710 - 722. [Abstract] [PDF]

				P. Cappello, T. Fraone, L. Barberis, C. Costa, E. Hirsch, A. R. Elia, C. Caorsi, T. Musso, F. Novelli, and M. Giovarelli CC-Chemokine Ligand 16 Induces a Novel Maturation Program in Human Immature Monocyte-Derived Dendritic Cells J. Immunol., November 1, 2006; 177(9): 6143 - 6151. [Abstract] [Full Text] [PDF]

				C. Guiducci, A. P. Vicari, S. Sangaletti, G. Trinchieri, and M. P. Colombo Redirecting In vivo Elicited Tumor Infiltrating Macrophages and Dendritic Cells towards Tumor Rejection Cancer Res., April 15, 2005; 65(8): 3437 - 3446. [Abstract] [Full Text] [PDF]