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Originally published online as doi:10.1189/jlb.0902474 on July 22, 2003

Published online before print July 22, 2003
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(Journal of Leukocyte Biology. 2003;74:952-958.)
© 2003 by Society for Leukocyte Biology

Different antigens trigger different Th1/Th2 reactions in neonatal mononuclear cells (MNCs) relating to T-bet/GATA-3 expression

Hong-Ren Yu, Jen-Chieh Chang, Rong-Fu Chen, Hau Chuang, Kuo-ching Hong, Lin Wang and Kuender D. Yang1

Division of Clinical Immunology & Allergy, Chang Gung Children’s Hospital and Graduate Institute of Clinical Medical Sciences, Chang Gung University, Kaohsing, Taiwan, R.O.C.

1Correspondence: Office of the Superintendents, Chang Gung Children’s Hospital, 123, Ta-Pei Road, Niao-Sung Hsiang, Kaohsiung Hsien 833, Taiwan. E-mail: yangkd{at}adm.cgmh.org.tw

Neonates are known to have poor cellular immunity, especially poor Th1 response. We investigated how neonatal mononuclear cells raised different Th1/Th2 reactions in response to different antigens. Employing Dermatophagoides pteronyssinus (Der p) extract and varicella zoster virus (VZV) as antigens, we assessed Th1/Th2 reactions as demonstrated by IL-4/IFN{gamma} production and mRNA expression, and transcriptional factors T-bet/GATA-3 mRNA expression in mononuclear cells from human umbilical cord blood (CBMC). Results showed that VZV induced a dramatic increase of IFN{gamma} production by adult peripheral blood mononuclear cells (PBMC), whereas VZV did not drive CBMC to release significant IFN{gamma} production (1614.7±362.0 vs. 49.0±29.3,p<0.005). However, Der p induced higher IFN{gamma} production by CBMC than VZV (298.1±171.8 vs. 49.0±29.3, P=0.047). In contrast, VZV did not induce significant IL-4 production either by CBMC or by PBMC. Der p induced a comparative IL-4 production by CBMC and PBMC (2.58±0.84 vs. 2.04±0.37, p>0.05). A real-time RT-PCR analysis of IL-4 and IFN{gamma} mRNA expression showed that VZV induced a significantly higher IFN{gamma}, but not IL-4, mRNA expression in PBMC than CBMC. Der p did not induce significant difference of IFN{gamma} or IL-4 mRNA expression in PBMC and CBMC. VZV enhanced Th1-related transcription factor T-bet mRNA expression, in association with later down-regulation of Th2-related GATA-3 mRNA expression in PBMC. However, VZV did not up-regulate T-bet or down-regulate GATA-3 expression significantly in CBMC. In contrast, Der p induced an early GATA-3 expression and later T-bet expression in CBMC. These results suggest that different antigens trigger various Th1/Th2 reactions in PBMC and CBMC resulting from kinetic changes of T-bet/GATA-3 expression.

Key Words: Neonate • Th1/Th2 • Dermatophagoides pteronyssinus (Der p) • varicella • IFN{gamma} • IL-4 • transcription factor




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