Journal of Leukocyte Biology
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Originally published online as doi:10.1189/jlb.0902433 on August 1, 2003

Published online before print August 1, 2003
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(Journal of Leukocyte Biology. 2003;74:846-856.)
© 2003 by Society for Leukocyte Biology

Phosphatidylserine expression and phagocytosis of apoptotic thymocytes during differentiation of monocytic cells

Melissa K. Callahan*, Margaret S. Halleck*, Stephen Krahling*, Andrew J. Henderson{dagger}, Patrick Williamson{ddagger} and Robert A. Schlegel*,1

* Department of Biochemistry and Molecular Biology,
{dagger} Department of Veterinary Sciences, Penn State University, University Park, PA 16802
{ddagger} Department of Biology, Amherst College, Amherst, MA 01002

1 Correspondence: Department of Biochemistry and Molecular Biology, 428 South Frear Laboratory, Penn State University, University Park, PA 16802. E-mail: ur3{at}psu.edu

Expression of phosphatidylserine (PS) on the surface of both macrophages and their apoptotic targets is required for efficient phagocytosis. Monocytes, the precursors of macrophages, do not express PS on their surface and do not efficiently phagocytose apoptotic cells. We report here that PS appears on the surface of both human monocytic U937 cells and primary human monocytes as they differentiate in culture and acquire the ability to phagocytose apoptotic thymocytes. Phagocytosis was blocked by pretreating either the apoptotic target or the phagocyte with annexin V to mask PS and was CD14-dependent. Expression of PS, like other events characteristic of differentiating monocytes such as Mac-1 expression, was independent of the agent used to induce differentiation and was insensitive to the addition of caspase inhibitors. These results demonstrate that PS is expressed on monocytes as part of their differentiation program and is independent of apoptosis.

Key Words: macrophages • annexin V • CD14 • Mac-1 • caspases




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