| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Published online before print August 21, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
HIV Immunopathogenesis Laboratory, The Wistar Institute, Philadelphia, Pennsylvania
1 Correspondence: The Wistar Institute, 3601 Spruce Street, Room 480, Philadelphia, PA 19130. E-mail: montaner{at}wistar.upenn.edu
Dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) has been described as an attachment molecule for human immunodeficiency virus type 1 (HIV-1) with the potential to mediate its transmission. We examined DC-SIGN expression in monocyte-derived macrophages (MDM) and its role in viral transmission when MDM were exposed to interleukin (IL)-13, IL-4, or interferon-
(IFN-). We show that IL-13 and IL-4 increase transcripts, total protein, and cell-surface expression of DC-SIGN in all MDM tested, IFN- results ranged from no change to up-regulation of surface expression, and message and total protein were, respectively, induced in all and 86% of donors tested. Transmission experiments of HIV-1 X4 between cytokine-treated MDM to Sup-T1 cells showed no association between total transmission and DC-SIGN up-regulation. IL-4 but not IL-13 resulted in a less than twofold increase in MDM viral transmission to CD4+ T cells in spite of a fourfold up-regulation in DC-SIGN expression by either cytokine. In contrast, IFN- treatment induced a decrease in total transmission by at least two-thirds, despite its induction of DC-SIGN. Soluble mannan resulted in a greater inhibition of viral transmission to CD4+ T cells than neutralizing anti-DC-SIGN monoclonal antibody (67–75% vs. 39–48%), supporting the role of mannose-binding receptors in viral transmission. Taken together, results show that DC-SIGN regulation in MDM does not singly predict the transmission potential of this cell type.
Key Words: IL-4 • IL-13 • IFN-
This article has been cited by other articles:
|
|
 |
|
  K.-F. Kong, K. Delroux, X. Wang, F. Qian, A. Arjona, S. E. Malawista, E. Fikrig, and R. R. Montgomery Dysregulation of TLR3 Impairs the Innate Immune Response to West Nile Virus in the Elderly J. Virol., August 1, 2008; 82(15): 7613 - 7623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Serrano-Gomez, R. T. Martinez-Nunez, E. Sierra-Filardi, N. Izquierdo, M. Colmenares, J. Pla, L. Rivas, J. Martinez-Picado, J. Jimenez-Barbero, J. L. Alonso-Lebrero, et al. AM3 Modulates Dendritic Cell Pathogen Recognition Capabilities by Targeting DC-SIGN Antimicrob. Agents Chemother., July 1, 2007; 51(7): 2313 - 2323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Saidi, G. Magri, C. Carbonneil, N. Nasreddine, M. Requena, and L. Belec IFN-{gamma}-activated monocytes weakly produce HIV-1 but induce the recruitment of HIV-sensitive T cells and enhance the viral production by these recruited T cells J. Leukoc. Biol., March 1, 2007; 81(3): 642 - 653. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Wahl, T. Greenwell-Wild, and N. Vazquez HIV accomplices and adversaries in macrophage infection J. Leukoc. Biol., November 1, 2006; 80(5): 973 - 983. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Rappocciolo, F. J. Jenkins, H. R. Hensler, P. Piazza, M. Jais, L. Borowski, S. C. Watkins, and C. R. Rinaldo Jr DC-SIGN Is a Receptor for Human Herpesvirus 8 on Dendritic Cells and Macrophages J. Immunol., February 1, 2006; 176(3): 1741 - 1749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Papasavvas, J. Sun, Q. Luo, E. C. Moore, B. Thiel, R. R. MacGregor, A. Minty, K. Mounzer, J. R. Kostman, and L. J. Montaner IL-13 Acutely Augments HIV-Specific and Recall Responses from HIV-1-Infected Subjects In Vitro by Modulating Monocytes J. Immunol., October 15, 2005; 175(8): 5532 - 5540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Granelli-Piperno, A. Pritsker, M. Pack, I. Shimeliovich, J.-F. Arrighi, C. G. Park, C. Trumpfheller, V. Piguet, T. M. Moran, and R. M. Steinman Dendritic Cell-Specific Intercellular Adhesion Molecule 3-Grabbing Nonintegrin/CD209 Is Abundant on Macrophages in the Normal Human Lymph Node and Is Not Required for Dendritic Cell Stimulation of the Mixed Leukocyte Reaction J. Immunol., October 1, 2005; 175(7): 4265 - 4273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Dominguez-Soto, A. Puig-Kroger, M. A. Vega, and A. L. Corbi PU.1 Regulates the Tissue-specific Expression of Dendritic Cell-specific Intercellular Adhesion Molecule (ICAM)-3-grabbing Nonintegrin J. Biol. Chem., September 30, 2005; 280(39): 33123 - 33131. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Marzi, T. Gramberg, G. Simmons, P. Moller, A. J. Rennekamp, M. Krumbiegel, M. Geier, J. Eisemann, N. Turza, B. Saunier, et al. DC-SIGN and DC-SIGNR Interact with the Glycoprotein of Marburg Virus and the S Protein of Severe Acute Respiratory Syndrome Coronavirus J. Virol., November 1, 2004; 78(21): 12090 - 12095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Serrano-Gomez, A. Dominguez-Soto, J. Ancochea, J. A. Jimenez-Heffernan, J. A. Leal, and A. L. Corbi Dendritic Cell-Specific Intercellular Adhesion Molecule 3-Grabbing Nonintegrin Mediates Binding and Internalization of Aspergillus fumigatus Conidia by Dendritic Cells and Macrophages J. Immunol., November 1, 2004; 173(9): 5635 - 5643. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Hu, I. Frank, V. Williams, J. J. Santos, P. Watts, G. E. Griffin, J. P. Moore, M. Pope, and R. J. Shattock Blockade of Attachment and Fusion Receptors Inhibits HIV-1 Infection of Human Cervical Tissue J. Exp. Med., April 19, 2004; 199(8): 1065 - 1075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Collman, C.-F. Perno, S. M. Crowe, M. Stevenson, and L. J. Montaner HIV and cells of macrophage/dendritic lineage and other non-T cell reservoirs: new answers yield new questions J. Leukoc. Biol., November 1, 2003; 74(5): 631 - 634. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
