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Published online before print May 22, 2003

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(Journal of Leukocyte Biology. 2003;74:102-110.)
© 2003 by Society for Leukocyte Biology

RNA analysis of B cell lines arrested at defined stages of differentiation allows for an approximation of gene expression patterns during B cell development

Panagiotis Tsapogas^*, Thomas Breslin, Sven Bilke, Anna Lagergren^*, Robert Månsson^*, David Liberg^*, Carsten Peterson and Mikael Sigvardsson^*

^* Laboratory for Cellular Differentiation, Department for Stemcell Biology, and
Department of Theoretical Physics, Lund University, Sweden

Correspondence: Mikael Sigvardsson, Laboratory for Cellular Differentiation, Department for Stemcell Biology, BMC B12, Lund, 22184, Sweden. E-mail: Mikael.Sigvardsson{at}stemcell.lu.se

The development of a mature B lymphocyte from a bone marrow stem cell is a highly ordered process involving stages with defined features and gene expression patterns. To obtain a deeper understanding of the molecular genetics of this process, we have performed RNA expression analysis of a set of mouse B lineage cell lines representing defined stages of B cell development using Affymetrix^TM microarrays. The cells were grouped based on their previously defined phenotypic features, and a gene expression pattern for each group of cell lines was established. The data indicated that the cell lines representing a defined stage generally presented a high similarity in overall expression profiles. Numerous genes could be identified as expressed with a restricted pattern using dCHIP-based, quantitative comparisons or presence/absence-based, probabilistic state analysis. These experiments provide a model for gene expression during B cell development, and the correctly identified expression patterns of a number of control genes suggest that a series of cell lines can be useful tools in the elucidation of the molecular genetics of a complex differentiation process.

Key Words: gene expression • immunoglobulin • progenitor B cells

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