Journal of Leukocyte Biology BioLegend: Treg, Th17, Stem Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published online as doi:10.1189/jlb.0602319 on May 22, 2003

Published online before print May 22, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jlb.0602319v1
73/6/802    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lutz, M. A.
Right arrow Articles by Correll, P. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lutz, M. A.
Right arrow Articles by Correll, P. H.
(Journal of Leukocyte Biology. 2003;73:802-814.)
© 2003 by Society for Leukocyte Biology

Activation of CR3-mediated phagocytosis by MSP requires the RON receptor, tyrosine kinase activity, phosphatidylinositol 3-kinase, and protein kinase C {zeta}

Michael A. Lutz and Pamela H. Correll

Department of Veterinary Science, Pennsylvania State University, Pathobiology Graduate Program, University Park

Correspondence: Dr. Pamela Correll, Department of Veterinary Science, 115 Henning Building, Pennsylvania State University, University Park, PA 16802. E-mail: phc7{at}psu.edu

Macrophage-stimulating protein (MSP) promotes the phagocytosis of C3bi-coated erythrocytes by resident peritoneal macrophages, although the mechanism by which this occurs is largely unknown. We show that MSP-induced complement-mediated phagocytosis requires the RON receptor tyrosine kinase and the {alpha}Mß2 integrin, as evidenced by the inability of RON-/- and {alpha}M-/- peritoneal macrophages to augment phagocytosis of complement-coated sheep erythrocytes in response to MSP. MSP stimulation of macrophages results in tyrosine phosphorylation and AKT activation, and inhibitor studies demonstrate a phagocytic requirement for tyrosine kinase and phosphatidylinositol 3-kinase (PI-3K) activity as well as activity of the atypical protein kinase C (PKC) isoform {zeta}, which localizes to MSP-induced phagosomes containing complement-coated beads. Additionally, MSP augments the ability of peritoneal macrophages to bind to intercellular adhesion molecule-1 (ICAM-1) via the {alpha}Mß2 integrin. MSP-induced ICAM-1 adhesion is also dependent on tyrosine kinase activity, PI-3K, and PKC {zeta}, indicating that these signaling requirements are upstream of complement receptor 3 activation.

Key Words: macrophage-stimulating protein • complement receptor 3 • intercellular adhesion molecule-1




This article has been cited by other articles:


Home page
 J. Immunol.Home page
C. B. Wilson, M. Ray, M. Lutz, D. Sharda, J. Xu, and P. A. Hankey
The RON Receptor Tyrosine Kinase Regulates IFN-{gamma} Production and Responses in Innate Immunity
J. Immunol., August 15, 2008; 181(4): 2303 - 2310.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Kalantari, O. F. Harandi, P. A. Hankey, and A. J. Henderson
HIV-1 Tat Mediates Degradation of RON Receptor Tyrosine Kinase, a Regulator of Inflammation
J. Immunol., July 15, 2008; 181(2): 1548 - 1555.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. G. Dupuy and E. Caron
Integrin-dependent phagocytosis - spreading from microadhesion to new concepts
J. Cell Sci., June 1, 2008; 121(11): 1773 - 1783.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Klatt, Z. Zhang, P. Kalantari, P. A. Hankey, D. S. Gilmour, and A. J. Henderson
The Receptor Tyrosine Kinase RON Represses HIV-1 Transcription by Targeting RNA Polymerase II Processivity
J. Immunol., February 1, 2008; 180(3): 1670 - 1677.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. A. Horan, K.-i. Watanabe, A. M. Kong, C. G. Bailey, J. E. J. Rasko, T. Sasaki, and C. A. Mitchell
Regulation of Fc{gamma}R-stimulated phagocytosis by the 72-kDa inositol polyphosphate 5-phosphatase: SHIP1, but not the 72-kDa 5-phosphatase, regulates complement receptor 3 mediated phagocytosis by differential recruitment of these 5-phosphatases to the phagocytic cup
Blood, December 15, 2007; 110(13): 4480 - 4491.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. L. Welm, J. B. Sneddon, C. Taylor, D. S. A. Nuyten, M. J. van de Vijver, B. H. Hasegawa, and J. M. Bishop
The macrophage-stimulating protein pathway promotes metastasis in a mouse model for breast cancer and predicts poor prognosis in humans
PNAS, May 1, 2007; 104(18): 7570 - 7575.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Sendide, N. E. Reiner, J. S. I. Lee, S. Bourgoin, A. Talal, and Z. Hmama
Cross-Talk between CD14 and Complement Receptor 3 Promotes Phagocytosis of Mycobacteria: Regulation by Phosphatidylinositol 3-Kinase and Cytohesin-1
J. Immunol., April 1, 2005; 174(7): 4210 - 4219.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. S. Lee, P. Kalantari, S. Tsutsui{section}, A. Klatt, J. Holden, P. H. Correll, C. Power{section}, and A. J. Henderson
RON Receptor Tyrosine Kinase, a Negative Regulator of Inflammation, Inhibits HIV-1 Transcription in Monocytes/Macrophages and Is Decreased in Brain Tissue from Patients with AIDS
J. Immunol., December 1, 2004; 173(11): 6864 - 6872.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
P. H. Correll, A. C. Morrison, and M. A. Lutz
Receptor tyrosine kinases and the regulation of macrophage activation
J. Leukoc. Biol., May 1, 2004; 75(5): 731 - 737.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the Society for Leukocyte Biology.