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Originally published online as doi:10.1189/jlb.1002483 on May 22, 2003

Published online before print May 22, 2003
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(Journal of Leukocyte Biology. 2003;73:756-763.)
© 2003 by Society for Leukocyte Biology

Prostaglandin E2 modulates dendritic cell function via EP2 and EP4 receptor subtypes

Hedi Harizi, Christophe Grosset and Norbert Gualde

Laboratoire d’Immunologie, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5540, Université de Bordeaux 2, Cedex, France

Correspondence: Dr. Harizi, Laboratoire d’Immunologie, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5540, Université de Bordeaux 2, 33076 Bordeaux Cedex, France. E-mail: harizi33{at}yahoo.fr

We have reported previously that PGE2 inhibits dendritic cells (DC) functions. Because E prostanoid receptor (EPR) subtypes involved in this action are unknown, expression and functions of these receptors were examined in DC. Western blot and flow cytometry analyses showed that all EPRs were coexpressed in DC. In a dose-dependent manner, lipopolysaccharide (LPS) enhanced EP2R/EP4R but not EP1R/EP3R expressions. NS-398, a cyclooxygenase (COX)-2-selective inhibitor, suppressed LPS-enhanced EP2R/EP4R expression, suggesting that COX-2-issued prostaglandin E2 (PGE2) modulates DC function through stimulation of specific EPR subtypes. Using selective agonists, we found that butaprost, an EP2R agonist, and PGE1 alcohol, an EP2R and EP2R/EP4R agonist, inhibited major histocompatibility complex class II expression and enhanced interleukin-10 production from DC. However, no effect was observed with sulprostone and 17-phenyl-{omega}-trinor-PGE2, selective agonists for EP1R and EP1R/EP3R, respectively. Treatment of DC with dibutyryl cyclic adenosine monophosphate (cAMP), an analog of cAMP, mimics PGE2-induced, inhibitory effects. Taken together, our data demonstrate that EP2R/EP4R are efficient for mediating PGE2-induced modulation of DC functions.

Key Words: PGE2 • EP receptors • agonists • cytokines




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