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Originally published online as doi:10.1189/jlb.0902443 on May 22, 2003

Published online before print May 22, 2003
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(Journal of Leukocyte Biology. 2003;73:731-738.)
© 2003 by Society for Leukocyte Biology

Ly49E expression points toward overlapping, but distinct, natural killer (NK) cell differentiation kinetics and potential of fetal versus adult lymphoid progenitors

Frederik Stevenaert, Katrien Van Beneden, An De Creus, Veronique Debacker, Jean Plum and Georges Leclercq

Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, University Hospital, Belgium

Correspondence: Dr. Georges Leclercq, Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, University Hospital, Blok A, 4th Floor, De Pintelaan 185, B-9000 Ghent, Belgium. E-mail: georges.leclercq{at}rug.ac.be

Using a new antibody, we found previously that contrary to adult natural killer (NK) cells, fetal NK cells have a unique phenotype, as they exclusively express Ly49E. This can be explained by an intrinsic different NK differentiation potential of fetal versus adult lymphoid progenitors, by immaturity of fetal NK cells or by instability of Ly49E expression. Here, we show that adult progenitor cells were still capable of differentiating into Ly49E-expressing NK cells but at a much lower frequency. Surprisingly, Ly49E expression in vitro did not require stromal cells. Kinetic analysis in vivo showed that Ly49E was expressed early, together with CD94/NKG2 and Ly49G2, followed by Ly49C, and finally Ly49D. Transfer of sorted Ly49E-positive fetal NK cells showed stable Ly49E expression, and later, part of these cells up-regulated other Ly49 members. These data indicate that although there are intrinsic differences, there is no strict fetal and adult wave of NK cell differentiation.

Key Words: cellular differentiation • repertoire development • cell-surface molecules




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