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* Division of Respiratory Medicine, Institute for Lung Health, University of Leicester Medical School, United Kingdom;
Laboratory for Allergy Transcriptome, RIKEN Research Centre for Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, Japan; and
Department of Dermatology, University of Kyoto Faculty of Medicine, Japan
Correspondence: Dr. Peter Bradding, Department of Respiratory Medicine, Glenfield Hospital, Institute for Lung Health, University of Leicester Medical School, Groby Rd., Leicester, LE3 9QP, UK. E-mail: pbradding{at}hotmail.com
Immunoglobulin E (IgE)-dependent activation of human mast cells (HMC) is characterized by an influx of extracellular calcium (Ca2+), which is essential for subsequent release of preformed (granule-derived) mediators and newly generated autacoids and cytokines. In addition, flow of ions such as K+ and Cl- is likely to play an important role in mast cell activation, proliferation, and chemotaxis through their effect on membrane potential and thus Ca2+ influx. It is therefore important to identify these critical molecular effectors of HMC function. In this study, we have used high-density oligonucleotide probe arrays to characterize for the first time the profile of ion channel gene expression in human lung, skin, and cord blood-derived mast cells. These cells express mRNA for inwardly rectifying and Ca2+-activated K+ channels, voltage-dependent Na+ and Ca2+ channels, purinergic P2X channels, transient receptor potential channels, and voltage-dependent and intracellular Cl- channels. IgE-dependent activation had little effect on ion channel expression, but distinct differences for some channels were observed between the different mast cell phenotypes, which may contribute to the mechanism of functional mast cell heterogeneity.
Key Words: Ca2+ • Na+ • K+ • Cl- • gene array
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