PeproTech Inc.

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baldini, P. M.
Right arrow Articles by Colizzi, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baldini, P. M.
Right arrow Articles by Colizzi, V.
(Journal of Leukocyte Biology. 2003;73:502-510.)
© 2003 by Society for Leukocyte Biology

Differential sensitivity of human monocytes and macrophages to ANP: a role of intracellular pH on reactive oxygen species production through the phospholipase involvement

P. M. Baldini, P. De Vito, A. Martino, M. Fraziano, C. Grimaldi, P. Luly, F. Zalfa and V. Colizzi

Department of Biology, University of Rome "Tor Vergata," Italy

Correspondence: Dr. P. M. Baldini, Department of Biology, University of Rome "Tor Vergata," Via della Ricerca Scientifica, 00133 Rome, Italy. E-mail: Baldini{at}uniroma2.it

Atrial natriuretic peptide (ANP), a cardiovascular hormone, elicits different biological actions in the immune system. The aim of the present work was to study the effect of ANP on the intracellular pH (pHi) of human monocytes and macrophages and to investigate whether pHi changes could play a role on phospholipase activities and reactive oxygen species (ROS) production. Human macrophages isolated by peripheral blood mononuclear cells and THP-1 monocytes, which were shown to express all three natriuretic peptide receptors (NPR-A, NPR-B, and NPR-C), were treated with physiological concentrations of ANP. A significant decrease of pHi was observed in ANP-treated macrophages with respect to untreated cells; this effect was paralleled by enhanced phospholipase activity and ROS production. Moreover, all assessed ANP effects seem to be mediated by the NPR-C. In contrast, no significant effect on pHi was observed in THP-1 monocytes treated with ANP. Treatment of macrophages or THP-1 monocytes with 5-(N-ethyl-N-isopropyl)amiloride, a specific Na+/H+ antiport inhibitor, decreases pHi in macrophages and monocytes. Our results indicate that only macrophages respond to ANP in terms of pHi and ROS production, through diacylglycerol and phosphatidic acid involvement, pointing to ANP as a new modulator of ROS production in macrophages.

Key Words: phosphatidic acid • diacyglycerol • NADPH oxidase




This article has been cited by other articles:


Home page
 IOVSHome page
E. M. Kniep, C. Roehlecke, N. Ozkucur, A. Steinberg, F. Reber, L. Knels, and R. H. W. Funk
Inhibition of Apoptosis and Reduction of Intracellular pH Decrease in Retinal Neural Cell Cultures by a Blocker of Carbonic Anhydrase.
Invest. Ophthalmol. Vis. Sci., March 1, 2006; 47(3): 1185 - 1192.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
H. Chon, C. A.J.M. Gaillard, B. B. van der Meijden, H. M. Dijstelbloem, R. J. Kraaijenhagen, D. van Leenen, F. C.P. Holstege, J. A. Joles, H. A.R. Bluyssen, H. A. Koomans, et al.
Broadly Altered Gene Expression in Blood Leukocytes in Essential Hypertension Is Absent During Treatment
Hypertension, May 1, 2004; 43(5): 947 - 951.
[Abstract] [Full Text] [PDF]