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* Department of Mediator and Signal Transduction Pharmacology, Kitasato University Graduate School of Medical Sciences, and
Department of Pharmacology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan
Correspondence: Yoshiteru Harada, Kitasato University, Mediator & Signal Transduction Pharmacology, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan. E-mail: yharada{at}ahs.kitasato-u.ac.jp
Granulocyte apoptosis and subsequent clearance by phagocytes are critical for the resolution of inflammation. However, no studies have addressed how the resolution proceeds in the inflammatory site. We studied the time course of neutrophil apoptosis and the following ingestion by mononuclear leukocytes in rat carrageenin-induced pleurisy, detecting DNA fragmentation by the deoxyuridine triphosphate-biotin nick-end labeling (TUNEL) method, by acridine orange staining, and from the DNA ladder pattern on electrophoresis. Neutrophil accumulation started 3–5 h after carrageenin injection and then maintained a plateau until 24 h. Neutrophils decreased steeply between days 1 and 3. Mononuclear leukocytes started to accumulate at 5 h and reached a peak at day 2. TUNEL-positive bodies and acridine orange-positive bodies first became detectable in the cytoplasm of the mononuclear leukocytes from 24 h and 9 h, respectively. Both methods indicated that mononuclear leukocytes containing fragmented DNA increased rapidly on days 1 and 2 and reached a peak at day 3. The characteristic ladder pattern of neutrophil DNA was observed from 5 h. Tumor necrosis factor 
was detectable on the start, and the levels of interleukin-10 and transforming growth factor-ß1 rose together with signs of neutrophil apoptosis and the following ingestion by mononuclear leukocytes. These results indicate that neutrophils start to undergo apoptosis just after the beginning of their accumulation in the inflammation site. Thus, evolution and resolution processes may proceed concurrently in acute inflammation.
Key Words: TUNEL • TNF- • interleukin • TGF-ß1
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