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(Journal of Leukocyte Biology. 2003;73:448-455.)
© 2003 by Society for Leukocyte Biology

Accelerated wound closure in neutrophil-depleted mice

Julia V. Dovi{dagger},*, Li-Ke He{dagger} and Luisa A. DiPietro*,{dagger}

Departments of
* Microbiology and Immunology and
{dagger} Surgery, Burn and Shock Trauma Institute, Loyola University Medical Center, Maywood, Illinois

Correspondence: Luisa A. DiPietro, Loyola University Medical Center, 2160 S. 1st Ave., BSTI, Building 110, Maywood, IL 60153. E-mail: ldipiet{at}lumc.edu

The infiltration of neutrophils into injured tissue is known to protect wounds from invading pathogens. However, more recent studies suggest that neutrophils might inhibit the wound repair process. To investigate the role of neutrophils in wounds, mice were neutrophil-depleted by injection with rabbit anti-mouse neutrophil serum. Remarkably, epidermal healing, measured by wound closure, proceeded significantly faster in neutropenic than control mice (77.7+14.2% vs. 41.2+0.9%, P<0.02 at day 2). Dermal healing was not affected by neutrophil depletion, as neither collagen deposition nor wound-breaking strength was significantly different between neutropenic and control mice. As the delayed repair of diabetic individuals exhibits robust inflammation, the effect of neutrophil depletion on diabetic wound healing was investigated. Similar to the observations in wild-type mice, wound closure was accelerated by nearly 50% in neutropenic, diabetic mice. The results suggest that although neutrophils may provide protection against infection, they may retard wound closure.

Key Words: healing • keratinocytes • inflammation • diabetic




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