T lymphocyte subsets defines unique tissue-specific functions
Department of Veterinary Molecular Biology, Montana State University, Bozeman
To elucidate the functions of circulating 
T cells, in the absence of antigen stimulation, the differential gene expression of two circulating T cell subsets was analyzed. The two subsets, with distinct trafficking phenotypes in young calves, were GD3.5+, CD8-, WC1+ or GD3.5-, CD2+, WC1-, and 90–100% CD8+ and were sorted based on GD3.5 and T cell receptor expression. Results from two different human arrays probed with cDNA from these T cell subsets indicated that they have markedly different tissue-specific functions. The genes preferentially expressed by GD3.5+ (CD8-) T cells demonstrated that they were highly activated, proliferative, and inflammatory, whereas those expressed by GD3.5- (primarily CD8+) T cells were involved in promoting quiescence, consistent with a role for T cells as sentinel mucosal cells, and several were interferon-regulated genes. Gene expression and phenotypic assays indicated that CD8+ T cells were apoptotic, whereas CD8- T cells were apoptosis-resistant. Differential expression of multiple genes was confirmed in both arrays: That of 14 genes was confirmed by quantitative reverse transcriptase-polymerase chain reaction and that of seven proteins was confirmed by flow cytometry. This novel, genomic analysis of circulating T cell subsets, without confounding effects of the tissue microenvironment, offers new insight into the biology and development of neonatal T cells.
Key Words: cDNA array • gene expression
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