| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
and expression of Pkare
* Laboratory of Molecular Immunology, Institute of Medical Technology, and
Department of Clinical Microbiology, Tampere University Hospital, University of Tampere, Finland;
Centre de Génetique Moléculaire et Cellulaire, Université Claude Bernard Lyon 1, Villeurbanne Cedex, France; and
Fred Hutchinson Cancer Research Center, Division of Basic Sciences, Seattle, Washington
Correspondence: Dr. Olli Silvennoinen, Institute of Medical Technology, University of Tampere Lenkkeilijänkatu 8, 33014 Tampere, Finland. E-mail: olli.silvennoinen{at}uta.fi
Macrophage-colony stimulating factor (M-CSF) regulates proliferation and differentiation of cells belonging to the monocytic lineage. We investigated the mechanisms of M-CSF differentiation signaling in follicular dendritic cell-P1 cells and analyzed the catalytic activation of different protein kinase C (PKC) isoforms. M-CSF induced rapid catalytic activation of PKC-
and membrane translocation of the tyrosine phosphorylated form of PKC-. Mutation of tyrosine 807 in the M-CSF receptor (Fms) abrogates cell differentiation but not a proliferative response to M-CSF, and FmsY807F failed to activate PKC-. We also investigated the downstream signaling pathways from PKC-. A cyclic adenosine monophosphate-regulated Ser/Thr kinase gene, protein kinase X (PRKX), has been associated with macrophage differentiation in human cells. We found that M-CSF and PKC- induced the expression of the PRKX murine homologue: PKA-related gene. Taken together, our results indicate that PKC- functions as a critical mediator of M-CSF-induced differentiation signaling.
Key Words: signal transduction • tyrosine phosphorylation • ser/thr kinase
This article has been cited by other articles:
|
|
 |
|
  B. Pakuts, C. Debonneville, L. M. Liontos, M. P. Loreto, and C. J. McGlade The Src-like Adaptor Protein 2 Regulates Colony-stimulating Factor-1 Receptor Signaling and Down-regulation J. Biol. Chem., June 22, 2007; 282(25): 17953 - 17963. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Pearn, J. Fisher, A. K. Burnett, and R. L. Darley The role of PKC and PDK1 in monocyte lineage specification by Ras Blood, May 15, 2007; 109(10): 4461 - 4469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Saijonmaa, T. Nyman, and F. Fyhrquist Atorvastatin inhibits angiotensin-converting enzyme induction in differentiating human macrophages Am J Physiol Heart Circ Physiol, April 1, 2007; 292(4): H1917 - H1921. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Li, Z.-X. Yu, and R. M. Kotin Profiles of PrKX Expression in Developmental Mouse Embryo and Human Tissues J. Histochem. Cytochem., August 1, 2005; 53(8): 1003 - 1009. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Wu, K. H. Van der Hoek, N. K. Ryan, R. J. Norman, and R. L. Robker Macrophage contributions to ovarian function Hum. Reprod. Update, March 1, 2004; 10(2): 119 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Li, Y.-J. Kim, C. Mantel, and H. E. Broxmeyer P-Selectin Enhances Generation of CD14+CD16+ Dendritic-Like Cells and Inhibits Macrophage Maturation from Human Peripheral Blood Monocytes J. Immunol., July 15, 2003; 171(2): 669 - 677. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
