HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bajer, A. A. Articles by Estes, D. M. Search for Related Content PubMed PubMed Citation Articles by Bajer, A. A. Articles by Estes, D. M.

(Journal of Leukocyte Biology. 2003;73:100-106.)
© 2003 by Society for Leukocyte Biology

Peripheral blood-derived bovine dendritic cells promote IgG1-restricted B cell responses in vitro

Anna A. Bajer^*, David Garcia-Tapia^*, Kimberly R. Jordan^*, Karen M. Haas, Dirk Werling, Chris J. Howard and D. Mark Estes^*

Departments of
^* Veterinary Pathobiology, Program for Prevention of Animal Infectious Diseases, and
Molecular Microbiology and Immunology, University of Missouri, Columbia;
Institute of Veterinary Virology, University of Bern, Switzerland; and
Institute for Animal Health, Compton, Newbury, United Kingdom

Correspondence: D. Mark Estes, Department of Veterinary Pathobiology, University of Missouri, 201 Connaway Hall, Columbia, MO 65211. E-mail: EstesD{at}missouri.edu

Regulation of humoral responses involves multiple cell types including the requirements for cognate interactions between T and B cells to drive CD40-dependent responses to T-dependent antigens. A third cell type has also been shown to play an essential role, the dendritic cell (DC). We demonstrate that bovine peripheral blood-derived (PB)-DC are similar in function to features described for human interstitial DC including the production of signature type 2 cytokines [interleukin (IL)-13, IL-10]. PB-DC express moderate-to-high costimulatory molecule expression, and major histocompatibility complex class II is negative for CD14 expression and has low or no expression of CD11c. Consistent with the interstitial phenotype is the ability of PB-DC to influence B cell activation and differentiation via direct expression of CD40L and type 2 cytokines. Collectively, these results suggest that direct B cell-DC interactions may promote an immunoglobulin-isotype expression pattern consistent with type 2 responses, independent of direct T cell involvement.

Key Words: isotype • immunoglobulin • cytokine • ruminant

This article has been cited by other articles:

				A. S. Austin, K. M. Haas, S. M. Naugler, A. A. Bajer, D. Garcia-Tapia, and D. M. Estes Identification and Characterization of a Novel Regulatory Factor: IgA-Inducing Protein J. Immunol., August 1, 2003; 171(3): 1336 - 1342. [Abstract] [Full Text] [PDF]