PeproTech Inc.

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bernardo, J.
Right arrow Articles by Simons, E. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bernardo, J.
Right arrow Articles by Simons, E. R.
(Journal of Leukocyte Biology. 2002;72:1172-1179.)
© 2002 by Society for Leukocyte Biology

Immune complex stimulation of human neutrophils involves a novel Ca2+/H+ exchanger that participates in the regulation of cytoplasmic pH: flow cytometric analysis of Ca2+/pH responses by subpopulations

John Bernardo, Hilary Hartlaub, Xin Yu, Heidi Long and Elizabeth R. Simons

Department of Biochemistry, Boston University School of Medicine, Massachusetts

Correspondence: Elizabeth R. Simons, Ph.D., or John Bernardo, M.D., R-304, Boston University School of Medicine, 80 E. Concord Street, Boston, MA 02118. E-mails: esimons@bu.edu or jbernardo{at}lung.bumc.bu.edu

The activation of human phagocytic leukocytes by immune complexes (IC) or opsonized microbes via their Fc and complement receptors has been well-described (for reviews, see refs. [1 2 3 ]). The mechanisms involved in this process are complex and depend on the receptors involved. The biochemical events that lead to the destruction of invading organisms in turn display varying degrees of interdependence, but the controlling elements that lead to the ultimate killing of ingested organisms within phagosomes by lysosomal enzymes and reactive oxygen intermediates are still not completely understood. We have addressed these mechanisms by following and correlating the kinetics of responses by individual cells, using multiparameter flow cytometry [3 4 ]. Using nonopsonized IC as stimuli, we document here the presence of a novel Ca2+/H+ voltage-independent channel in human neutrophils, which helps to control their cytoplasmic pH.

Key Words: calcium channels • Fc receptors • pH regulation • flow cytometry




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
A. J. Sandoval, J. P. Riquelme, M. D. Carretta, J. L. Hancke, M. A. Hidalgo, and R. A. Burgos
Store-operated calcium entry mediates intracellular alkalinization, ERK1/2, and Akt/PKB phosphorylation in bovine neutrophils
J. Leukoc. Biol., November 1, 2007; 82(5): 1266 - 1277.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. M. Herrmann, J. Bernardo, H. J. Long, K. Seetoo, M. E. McMenamin, E. L. Batista Jr., T. E. Van Dyke, and E. R. Simons
Sequential Chemotactic and Phagocytic Activation of Human Polymorphonuclear Neutrophils
Infect. Immun., August 1, 2007; 75(8): 3989 - 3998.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
J. M. Herrmann, A. Kantarci, H. Long, J. Bernardo, H. Hasturk, L. V. Wray Jr., E. R. Simons, and T. E. Van Dyke
Simultaneous measurements of cytoplasmic Ca2+ responses and intracellular pH in neutrophils of localized aggressive periodontitis (LAP) patients
J. Leukoc. Biol., September 1, 2005; 78(3): 612 - 619.
[Abstract] [Full Text] [PDF]